{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T22:51:42Z","timestamp":1777503102608,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dengue virus (DENV) induced severe manifestations is a precursor for fatality among infected patients. Previous autopsy examinations of severe dengue (SD) patients reported presence of apoptotic cells in liver, brain, intestinal and lung tissues. Thus, serum\u2010level of major apoptotic proteins of dengue patients was evaluated in the current study, along with their biochemical parameters. Patients were categorized according to World Health Organization (WHO)\u2010defined classification. DENV\u2010infection was screened among 165 symptomatic patients by quantitative reverse transcription polymerase chain reaction, antidengue IgM, and IgG ELISA. Serum levels of apoptotic (Capase\u20103,7,8, Bcl\u20102 and FasL) and hepatic\u2010markers, lipid profile, hematological parameters of 78 dengue\u2010positive patients were determined by sandwich\u2010ELISA\/immunoturbidimetry\/auto\u2010analyzer. Significantly higher levels of caspase\u20103,7,8 and FasL was detected among SD patients compared to those without warning (WOW) signs. Amongst biochemical parameters, bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase serum concentrations significantly increased among severe patients. Principal component analysis followed by hierarchical clustering differentiated severe and with warning dengue patient groups from those WOW using caspase\u20103,7,8 and FasL biomarkers\u2014thus clearly distinguishing severe\u2010dengue group. Correlation analyses also established strong positive correlation between caspase\u20103,7,8 and FasL. Thus, serum level of caspase\u20103,7,8 and FasL during early stage of infection could be used as biomarkers for WHO\u2010defined dengue disease severity.<\/jats:p>","DOI":"10.1002\/jmv.29180","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T13:45:35Z","timestamp":1697723135000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Impact of apoptotic biomarkers for prognosis of dengue disease severity among eastern Indian patients"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0041-3465","authenticated-orcid":false,"given":"Siddhartha","family":"Sengupta","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Medical Biotechnology Calcutta School of Tropical Medicine Kolkata West Bengal 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