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Notably, 90% of HBV\u2010positive HCC cases exhibit detectable HBV integrations, hinting at the potential early entanglement of these viral integrations in tumorigenesis and their subsequent oncogenic implications. Nevertheless, the precise chronology of integration events during HCC tumorigenesis, alongside their sequential structural patterns, has remained elusive thus far. In this study, we applied whole\u2010genome sequencing to multiple biopsies extracted from six HBV\u2010positive HCC cases. Through this approach, we identified point mutations and viral integrations, offering a blueprint for the intricate tumor phylogeny of these samples. The emergent narrative paints a rich tapestry of diverse evolutionary trajectories characterizing the analyzed tumors. We uncovered oncogenic integration events in some samples that appear to happen before and during the initiation stage of tumor development based on their locations in reconstituted trajectories. Furthermore, we conducted additional long\u2010read sequencing of selected samples and unveiled integration\u2010bridged chromosome rearrangements and tandem repeats of the HBV sequence within integrations. In summary, this study revealed premalignant oncogenic and sequential complex integrations and highlighted the contributions of HBV integrations to HCC development and genome instability.<\/jats:p>","DOI":"10.1002\/jmv.29187","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T13:54:38Z","timestamp":1698242078000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Temporal and structural patterns of hepatitis B virus integrations in hepatocellular carcinoma"],"prefix":"10.1002","volume":"95","author":[{"given":"Haozhen","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery The Affiliated Drum Tower Hospital of Nanjing University Medical School  Nanjing China"},{"name":"Hepatobiliary Institute, Nanjing University  Nanjing 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