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Here, we developed a novel method based on recombinase polymerase amplification (RPA) combined with CRISPR\u2010Cas12a to detect three viral pathogens, including SARS\u2010CoV\u20102, influenza A, and influenza B, which cause similar symptom complexes of flu cold in the respiratory tract. The detection method can be completed within 1\u2009h, which is faster than other standard detection methods, and the limit of detection is approximately 10<jats:sup>2<\/jats:sup> copies\/\u03bcL. Additionally, this detection system is highly specific and there is no cross\u2010reactivity with other common respiratory tract pathogens. Based on this assay, we further developed a more simplified RPA\/CRISPR\u2010Cas12a system combined with lateral flow assay on a manual microfluidic chip, which can simultaneously detect these three viruses. This low\u2010cost detection system is rapid and sensitive, which could be applied in the field and resource\u2010limited areas without bulky and expensive instruments, providing powerful tools for the point\u2010of\u2010care diagnostic.<\/jats:p>","DOI":"10.1002\/jmv.29215","type":"journal-article","created":{"date-parts":[[2023,11,7]],"date-time":"2023-11-07T13:36:36Z","timestamp":1699364196000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Development of a rapid, sensitive detection method for SARS\u2010CoV\u20102 and influenza virus based on recombinase polymerase amplification combined with CRISPR\u2010Cas12a assay"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6310-6837","authenticated-orcid":false,"given":"Yuning","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Reagent Research and Development Shenzhen YHLO Biotech Co., Ltd. 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