{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:13:59Z","timestamp":1792174439541,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      The introduction of primary human papillomavirus (HPV) cervical cancer screening requires the implementation of an appropriate triage strategy that will be effective in detecting high\u2010grade cervical disease without losing diagnostic specificity. From the 30.066 screening tests results, a total of 1086 with available high\u2010risk human papillomavirus (HRHPV) with limited genotyping, cytology, and p16\/Ki67 dual\u2010stain were selected. Two triage strategies for primary HPV screening were analyzed retrospectively based on the study group. Performance characteristics for p16\/Ki67 and cytology triage in the detection of cervical intraepithelial neoplasia grade 2 or worse (CIN2+) and grade 3 or worse (CIN3+) were calculated, detected in colposcopic biopsy. In HPV16\/18\u2010positive cases, primary HPV with p16\/Ki67 triage was significantly more specific than cytology (53.1%\/16.8% for CIN2+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001; 45.9%\/17.0% for CIN3+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), with yielded sensitivity (95.7%\/84.8% for CIN2+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0955; 100.0%\/87.5% for CIN3+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0832). In other HRHPV\u2010positive cases (N16\/N18), p16\/Ki67 triage was also significantly higher specific (51.3%\/15.3% for CIN2+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001; 44.5%\/16.5% for CIN3+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), with sensitivity (92.3%\/74.4% for CIN2+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0522; 90.9%\/81.8% for CIN3+;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.5637). Diagnostic predictive values were significantly higher for p16\/Ki67 triage with the highest PPV in HPV16\/18\u2010positive cases for CIN2+ (45.4%; 95% confidence interval [CI]: 35.2\u201355.8;\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001) and very high NPV in all HPV\u2010positive cases regardless of detected genotype (96.3%\u2013100.0%). The risk (1\u2010NPV) for CIN3+ in HRHPV16\/18\u2010positive\/p16\/Ki67\u2010negative women was 0.0%. Superior diagnostic performance compared to cytology for detecting cervical cancer precursors indicates that p16\/Ki67 dual\u2010immunostain may be a highly effective tool of triage in primary HPV screening with limited HPV 16\/18 genotyping in secondary cervical cancer prevention.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.29271","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T18:47:16Z","timestamp":1701110836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["p16\/Ki67 dual stain triage versus cytology in primary human papillomavirus\u2010based cervical cancer screening with limited genotyping"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9971-1093","authenticated-orcid":false,"given":"Martyna","family":"Trzeszcz","sequence":"first","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"},{"name":"Division of Pathology and Clinical Cytology University Hospital in Wroclaw Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2152-157X","authenticated-orcid":false,"given":"Maciej","family":"Mazurec","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5512-763X","authenticated-orcid":false,"given":"Robert","family":"Jach","sequence":"additional","affiliation":[{"name":"Division of Gynecologic Endocrinology Jagiellonian University Medical College Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karolina","family":"Mazurec","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela","family":"Kotkowska\u2010Szeps","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdalena","family":"Kania","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariola","family":"Wantuchowicz","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"Wasowska","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Duczek\u2010Polakiewicz","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrycja","family":"Rozmus","sequence":"additional","affiliation":[{"name":"Corfamed Woman's Health Center Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2147-7177","authenticated-orcid":false,"given":"Joanna","family":"Streb","sequence":"additional","affiliation":[{"name":"Department of Oncology Jagiellonian University Medical College Krakow Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4240-7899","authenticated-orcid":false,"given":"Agnieszka","family":"Halon","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Pathology, Division of Clinical Pathology Wroclaw Medical University Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2019.09.006"},{"key":"e_1_2_9_3_1","volume-title":"MSAC Outcomes. 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