{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:49:56Z","timestamp":1786978196909,"version":"build-2736575974"},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Human immunodeficiency virus (HIV) capsid is one of the most recent viral proteins successfully targeted for the development of antiretrovirals. Lenacapavir is a first in class HIV\u20101 capsid inhibitor that was recently approved for the treatment of highly treatment\u2010experienced people with HIV in combination with other anti\u2010HIV drugs. Owing to the novelty of the viral target, methods to characterize the potential resistance\u2010associated mutations present in capsid upon treatment failure have not been fully established yet. Here, we describe a rapid and simple method to amplify capsid fragments and to determine their sequence from various clinical samples including diverse HIV\u20101 subtypes. These methods could easily be implemented in laboratories, including hospital laboratories often caring for this patient population.<\/jats:p>","DOI":"10.1002\/jmv.29292","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T11:19:51Z","timestamp":1702034391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Rapid HIV\u20101 genotyping assay for the detection of capsid mutations"],"prefix":"10.1002","volume":"95","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0911-4237","authenticated-orcid":false,"given":"Nicolas","family":"Margot","sequence":"first","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidula","family":"Naik","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anurag","family":"Nekkalapudi","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archana","family":"Boopathy","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brie","family":"Falkard","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Callebaut","sequence":"additional","affiliation":[{"name":"Gilead Sciences Inc. Clinical Virology Foster City California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,12,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2443-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2352-3018(23)00113-3"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2115542"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiac364"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","unstructured":"OgbuaguO Segal\u2010MaurerS RatanasuwanW et al. Efficacy and safety of long\u2010acting subcutaneous lenacapavir in heavily treatment\u2010experienced people with multi\u2010drug resistant HIV: Week 52 results (Oral presentation 1585). Paper presented at: Infectious Diseases Society of America ID Week; October 19\u201023 2022;Washington DC USA.","DOI":"10.1093\/ofid\/ofac492.108"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02057-20"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa060"},{"key":"e_1_2_8_9_1","unstructured":"YantSR MulatoA HansenD et al. In vitro resistance profile of GS\u20106207 a first\u2010in\u2010class picomolar HIV capsid inhibitor in clinical development as a novel long\u2010acting antiretroviral agent (Abstract No. TUPE075). Paper presented at: 10th IAS Conference on HIV Science (IAS 2019); July 21\u201024 2019; Mexico City Mexico."},{"issue":"4","key":"e_1_2_8_10_1","first-page":"559","article-title":"2022 update of the drug resistance mutations in HIV\u20101","volume":"30","author":"Wensing AM","year":"2022","journal-title":"Top Antivir Med"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29292","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,6]],"date-time":"2024-05-06T11:05:54Z","timestamp":1714993554000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2023,12]]}},"alternative-id":["10.1002\/jmv.29292"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29292","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,12]]},"assertion":[{"value":"2023-06-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29292"}}