{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T15:08:18Z","timestamp":1782918498383,"version":"3.54.5"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T00:00:00Z","timestamp":1703635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Utilizing multiplex real time polymerase chain reaction (RT\u2010PCR) for rapid diagnosis of gastroenteritis, enables simultaneous detection of multiple pathogens. A comparative analysis of disease characteristics was conducted between cases with single and multiple viruses. Rotavirus vaccine was introduced in 2010, reaching a 70% coverage in 2 years. All rectal swabs collected from diarrheic children (&lt;5 years) between December 2017 and March 2022 were included. Detection of the same viruses within 2 months was considered a single episode. Episodes with positive stool bacterial PCR were excluded. A total of 5879 samples were collected, revealing 86.9% (1509) with single virus detection and 13.1% (227) with multiple viruses. The most frequent combination was rotavirus and norovirus (27.8%), these infections followed a winter\u2010spring seasonality akin to rotavirus. Children with multivirus infections exhibited higher immunodeficiency (OR 2.06) rates, but lower food allergy (OR 0.45) and prematurity rates (OR 0.55) compared to single infections. Greater disease severity, evaluated by the Vesikari score, was observed in multivirus episodes (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001, OR 1.12). Multivirus infections accounted for 13.1% of symptomatic cases in hospitalized young children. Despite vaccination efforts, rotavirus remained prominent, frequently in co\u2010infections with norovirus. Overall, multivirus infections were linked to more severe diseases than single virus cases.<\/jats:p>","DOI":"10.1002\/jmv.29344","type":"journal-article","created":{"date-parts":[[2023,12,27]],"date-time":"2023-12-27T10:09:49Z","timestamp":1703671789000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparing single versus\u00a0multiple virus detection in pediatric acute gastroenteritis postimplementation of routine multiplex RT\u2010PCR diagnostic testing"],"prefix":"10.1002","volume":"96","author":[{"given":"Guy","family":"Hazan","sequence":"first","affiliation":[{"name":"Pediatric Department D. Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University  Beer Sheva Israel"},{"name":"Pediatric Pulmonary Unit, Soroka University Medical Center, Faculty of Health Sciences Ben Gurion University  Beer Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoav","family":"Goldstein","sequence":"additional","affiliation":[{"name":"Pediatric Department D. Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University  Beer Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Greenberg","sequence":"additional","affiliation":[{"name":"Pediatric Infectious Diseases Unit, Soroka University Medical Center, Faculty of Health Sciences Ben Gurion University  Beer Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firas","family":"Khalde","sequence":"additional","affiliation":[{"name":"Pediatric Department D. 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