{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:39Z","timestamp":1778544039118,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,4]],"date-time":"2024-01-04T00:00:00Z","timestamp":1704326400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Orthohantaviruses, etiological agents of hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome, pose a critical public health threat worldwide. <jats:italic>Hantaan orthohantavirus<\/jats:italic> (HTNV) outbreaks are particularly endemic in Gyeonggi Province in northern area of the Republic of Korea (ROK). Small mammals were collected from three regions in the Gyeonggi Province during 2017 and 2018. Serological and molecular prevalence of HTNV was 25\/201 (12.4%) and 10\/25 (40%), respectively. A novel nanopore\u2010based diagnostic assay using a cost\u2010efficient Flongle chip was developed to rapidly and sensitively detect HTNV infection in rodent specimens within 3\u2009h. A rapid phylogeographical surveillance of HTNV at high\u2010resolution phylogeny was established using the amplicon\u2010based Flongle sequencing. In total, seven whole\u2010genome sequences of HTNV were newly obtained from wild rodents collected in Paju\u2010si (Gaekhyeon\u2010ri) and Yeoncheon\u2010gun (Hyeonga\u2010ri and Wangnim\u2010ri), Gyeonggi Province. Phylogenetic analyses revealed well\u2010supported evolutionary divergence and genetic diversity, enhancing the resolution of the phylogeographic map of orthohantaviruses in the ROK. Incongruences in phylogenetic patterns were identified among HTNV tripartite genomes, suggesting differential evolution for each segment. These findings provide crucial insights into on\u2010site diagnostics, genome\u2010based surveillance, and the evolutionary dynamics of orthohantaviruses to mitigate hantaviral outbreaks in HFRS\u2010endemic areas in the ROK.<\/jats:p>","DOI":"10.1002\/jmv.29346","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T06:53:20Z","timestamp":1704437600000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["High\u2010resolution phylogeographical surveillance of <i>Hantaan orthohantavirus<\/i> using rapid amplicon\u2010based Flongle sequencing, Republic of Korea"],"prefix":"10.1002","volume":"96","author":[{"given":"Jongwoo","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology Korea University College of Medicine Seoul Republic of Korea"},{"name":"BK21 Graduate Program Korea University College of Medicine  Seoul Republic of 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