{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T18:14:57Z","timestamp":1776104097786,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T00:00:00Z","timestamp":1705536000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although neonates are commonly exposed to vaginal herpes simplex virus (HSV)\u20102, neonatal herpes is rare. Therefore, we analyzed paired infant and maternal HSV\u20102 isolates from two cases of mother\u2010to\u2010infant transmission to identify viral factors contributing to vertical transmission. Sixteen infant isolates with neonatal herpes and 27 genital isolates in their third trimester were included. The infant isolates were significantly more temperature\u2010independent than the maternal isolates. Sequence comparison revealed viral UL13 protein kinase (UL13\u2010PK) mutation in the infant isolates in both cases. In the expanded cohort, infant isolates (5\/18) had significantly more UL13\u2010PK mutations than genital isolates (1\/29). Isolates within 8 days post\u2010birth (3\/4) had a significantly higher frequency of UL13\u2010PK mutation than those after 9 days (2\/14), suggesting a close association between UL13\u2010PK mutations and vertical transmission. Elongation factor 1\u2010delta was identified as a target of UL13\u2010PK by proteomic analysis of UL13\u2010PK\u2010positive and \u2010negative HepG2 cells. The mixed infant isolates with the intact and mutated UL13\u2010PK conferred altered cell tropism, temperature independence adapting to fetal temperature, and better growth properties in Vero and hepatoblastoma HepG2 cells than in HSV\u20102 with intact and mutated UL13\u2010PK alone, indicating that viral UL13\u2010PK mutation is essential for vertical HSV\u20102 transmission.<\/jats:p>","DOI":"10.1002\/jmv.29379","type":"journal-article","created":{"date-parts":[[2024,1,18]],"date-time":"2024-01-18T10:50:37Z","timestamp":1705575037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Vertical mother\u2010to\u2010infant transmission of herpes simplex virus 2 is correlated with tropism due to mutations in viral UL13"],"prefix":"10.1002","volume":"96","author":[{"given":"Kimiyasu","family":"Shiraki","sequence":"first","affiliation":[{"name":"Department of Nursing Senri Kinran University, Suita  Suita Osaka 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