{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:02:48Z","timestamp":1791162168757,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the interference of lipemia on measurement of HBsAg, anti\u2010HBs, HBeAg, anti\u2010HBe, anti\u2010HBc, anti\u2010HCV, HIV Ag\/Ab, and anti\u2010TP in serum by chemiluminescent microparticle immunoassay (CMIA) and compare lipemia removing performance between high\u2010speed centrifugation and Lipoclear reagent. Mixed native serum samples (NSs) and hyperlipemia serum samples (HLS) were prepared for the investigated parameters. The levels of these parameters in NS and HLS were determined by CMIA on an Abbott ARCHITECT i2000SR immunoassay analyzer. HBsAg, anti\u2010HBs, and anti\u2010TP were affected with relative bias &gt;12.5% (acceptable limit) when the level of triacylglycerol (TG) was higher than 27.12\u2009mmol\/L in HLS. Clinically unacceptable bias were observed for HBeAg and anti\u2010HBe in HLS with TG higher than 40.52\u2009mmol\/L. However, anti\u2010HCV and HIV Ag\/Ab were not interfered in severe lipemia with TG\u2009&lt;\u200952.03\u2009mmol\/L. In addition, the Lipoclear reagent did not reduce the interference of lipemia with relative bias from \u221262.50% to \u221218.02%. The high\u2010speed centrifugation under the optimized condition of 12\u2009000\n                    <jats:italic>g<\/jats:italic>\n                    for 10\u2009min successfully removed the interference of lipemia with relative bias from \u22125.93% to 0% for HBsAg, anti\u2010HBs, HBeAg, anti\u2010HBe, anti\u2010HBc, and anti\u2010TP. To conclude, high\u2010speed centrifugation can be used for removing the interference of lipemia to measure HBsAg, anti\u2010HBs, HBeAg, anti\u2010HBe, anti\u2010HBc, and anti\u2010TP. Accordingly, a standardized sample preanalytical preparation of the patients and other screening participants as well as a specimen examination procedure for removing lipemia interference on the serological tests was recommended.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29385","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T02:13:39Z","timestamp":1707272019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["High\u2010speed centrifugation rather than Lipoclear reagent can be used for removing the interference of lipemia on serological tests of infectious diseases: AIDS, hepatitis B, hepatitis C, and syphilis by chemiluminescent microparticle immunoassay"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9548-0654","authenticated-orcid":false,"given":"Weiping","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Zigong First People's Hospital  Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Sichuan Academy of Medical Sciences &amp; Sichuan Provincial People's Hospital University of Electronic Science and Technology of China  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minggang","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Zigong First People's Hospital  Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengjian","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Zigong First People's Hospital  Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9527-9136","authenticated-orcid":false,"given":"Yaohui","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Zigong First People's Hospital  Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7897-9923","authenticated-orcid":false,"given":"Xia","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Zigong First People's Hospital  Zigong Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-1288"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plabm.2021.e00232"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638719858690"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.06.020"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/40.11.1996"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2014.008"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2013.08.015"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.5772\/35797"},{"key":"e_1_2_10_10_1","unstructured":"Lipoclear. Accessed October 27 2013.http:\/\/www.statspin.com\/pdfs\/64-003957-001D.pdf"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2011.016"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/9783110266221"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2011.025"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2014.01.001"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.004"},{"issue":"1","key":"e_1_2_10_16_1","article-title":"Elimination of lipaemic interference by high\u2010speed centrifugation","volume":"33","author":"Sol\u00e9\u2010Enrech G","year":"2023","journal-title":"Biochem Med (Zagreb)"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2018.38.6.518"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29385","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29385","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29385","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:25:15Z","timestamp":1772987115000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29385"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29385"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29385","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29385"}}