{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T15:24:48Z","timestamp":1788621888811,"version":"build-2803163510"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cross\u2010neutralizing activity of human antibody response against Dengue virus complex (DENV) changes importantly over time. Domain III (DIII) of the envelope protein of DENV elicits a potently neutralizing and mostly type\u2010specific IgG response. We used sera from 24 individuals from early\u2010 or late convalescence of DENV1 infection to investigate the evolution of anti\u2010DIII human IgG with the time lapse since the infection. We evaluated the correlation between the serotype\u2010specific reactivity against recombinant DIII proteins and the neutralization capacity against the four serotypes, and examined its behavior with the time of convalescence. Also, we use a library of 71 alanine mutants of surface\u2010exposed amino acid residues to investigate the dominant epitopes. In early convalescence anti\u2010DIII titers and potency of virus neutralization were positively associated with correlation coefficients from 0.82 to 1.0 for the four serotypes. For late convalescence, a positive correlation (\n                    <jats:italic>r<\/jats:italic>\n                    \u2009=\u20090.69) was found only for DENV1. The dominant epitope of the type\u2010specific response is centered in the FG\u2010loop (G383, E384, and K385) and includes most of the lateral ridge. The dominant epitope of the anti\u2010DIII cross\u2010reactive IgG in secondary infections shifts from the A\u2010strand during early convalescence to a site centered in residues E314\u2010H317 of the AB\u2010loop and I352\u2010E368 of the DI\/DIII interface, in late convalescence. An immunoassay based on the detection of IgG anti\u2010DIII response can be implemented for detection of infecting serotype in diagnosis of DENV infection, either primary or secondary. Human dominant epitopes of the cross\u2010reactive circulating antibodies change with time of convalescence.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29443","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T13:18:10Z","timestamp":1708348690000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Dominant epitopes of cross\u2010reactive anti\u2010domain III human antibody response change from early to late convalescence of infection with dengue virus"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4375-4342","authenticated-orcid":false,"given":"Luis Gabriel","family":"Gonz\u00e1lez\u2010Lodeiro","sequence":"first","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7291-2274","authenticated-orcid":false,"given":"Alejandro","family":"Mart\u00edn Dunn","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9073-6198","authenticated-orcid":false,"given":"Dayron","family":"Mart\u00edn Prieto","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7334-4758","authenticated-orcid":false,"given":"Danya","family":"Medina\u2010Carrasco","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisandra E.","family":"Garc\u00eda de Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Maldonado Bauz\u00e1","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"},{"name":"Faculty of Biology student University of Havana Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glay","family":"Chinea Santiago","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6731-1757","authenticated-orcid":false,"given":"Vivian","family":"Huerta Galindo","sequence":"additional","affiliation":[{"name":"Department of Systems Biology, Biomedical Research Center for Genetic Engineering and Biotechnology Havana Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60572-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.55"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.8.20718"},{"key":"e_1_2_11_5_1","unstructured":"WHO. World Health Organization: dengue and severe dengue. 2023.www.who.int\/healt-topics\/dengue-and-severe-dengue#tab=_1"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929866525666180709115506"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-017-0010-8"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.2.1223-1231.2005"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6311-z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2015.12.004"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005395"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-013-1956-4"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2022.02.070"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.12.020"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00432-07"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00606-08"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.068544-0"},{"key":"e_1_2_11_18_1","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1093\/nar\/gks1195","article-title":"Genbank","volume":"41","author":"Benson DA","year":"2013","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_11_19_1","unstructured":"ChineaG HuertaV MartinAM et al. WO2015131858A2. Beta hairpin peptides with antiviral properties against dengue virus. 2015."},{"key":"e_1_2_11_20_1","article-title":"A time\u2010efficient workflow to purify a library of alanine mutants of surface\u2010exposed residues of the domain III of the envelope protein of dengue virus serotype 1","author":"Martin Prieto D","year":"2023","journal-title":"MicroPubl Biol"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.235"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh435"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-8822-y"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.06.037"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2023.109883"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28700"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110740"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abk0058"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.572681"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006191"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112304"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20143464"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2017.03.004"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.08.011"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001447"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.06.044"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300064"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01372"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.055178-0"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06871-11"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01023-18"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscombsci.0c00073"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007996"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986612800191026"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2014.11.009"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004991"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2009.03.016"},{"key":"e_1_2_11_49_1","unstructured":"World Health Organization. Special Programme for Research and Training in Tropical Diseases World Health Organization eds. Dengue: Guidelines for Diagnosis Treatment Prevention and Control. New ed. TDR: World Health Organization. 2009."},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12502"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903181106"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29443","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29443","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:23:56Z","timestamp":1772987036000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29443"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":50,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29443"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29443","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-11-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29443"}}