{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T09:50:51Z","timestamp":1785750651998,"version":"3.56.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Membrane\u2010associated RING\u2010CH (MARCH) family proteins were recently reported to inhibit viral replication through multiple modes. Previous work showed that human MARCH8 blocked Ebola virus (EBOV) glycoprotein (GP) maturation. Our study here demonstrates that human MARCH1 and MARCH2 share a similar pattern to MARCH8 in restricting EBOV GP\u2010pseudotyped viral infection. Human MARCH1 and MARCH2 retain EBOV GP at the\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010Golgi network, reduce its cell surface display, and impair EBOV GP\u2010pseudotyped virions infectivity. Furthermore, we uncover that the host proprotein convertase\u00a0furin could interact with human MARCH1\/2 and EBOV GP intracellularly. Importantly, the furin P domain is verified to be recognized by MARCH1\/2\/8, which is critical for their blocking activities. Besides, bovine MARCH2 and murine MARCH1 also impair EBOV GP proteolytic processing. Altogether, our findings confirm that MARCH1\/2 proteins of different mammalian origins showed a relatively conserved feature in blocking EBOV GP cleavage, which could provide clues for subsequent MARCHs antiviral studies and may facilitate the development of novel strategies to antagonize enveloped virus infection.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29445","type":"journal-article","created":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T07:14:51Z","timestamp":1706771691000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Human MARCH1, 2, and 8 block Ebola virus envelope glycoprotein cleavage via targeting furin P domain"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9548-7151","authenticated-orcid":false,"given":"Changqing","family":"Yu","sequence":"first","affiliation":[{"name":"Engineering Center of Agricultural Biosafety Assessment and Biotechnology School of Advanced Agricultural Sciences, Yibin Vocational and Technical College  Yibin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanzhe","family":"Bai","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Harbin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Tan","sequence":"additional","affiliation":[{"name":"College of Advanced Agriculture and Ecological Environment Heilongjiang University  Harbin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Bai","sequence":"additional","affiliation":[{"name":"College of Animal Science Wenzhou Vocational College of Science and Technology  Wenzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Center of Agricultural Biosafety Assessment and Biotechnology School of Advanced Agricultural Sciences, Yibin Vocational and Technical College  Yibin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Heilongjiang Bayi Agricultural University  Daqing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingbo","family":"Zhai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Zoonose Prevention and Control at Universities of Inner Mongolia Autonomous Region, Medical College Inner Mongolia Minzu University  Tongliao People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengzhou","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan\u2010Dong","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Animal Disease Control and Prevention Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences  Harbin People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8797-1322","authenticated-orcid":false,"given":"Chunfu","family":"Zheng","sequence":"additional","affiliation":[{"name":"Institute of Animal Health Guangdong Academy of Agricultural Sciences, Key Laboratory of Livestock Disease Prevention of Guangdong Province, Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province, Ministry of Agriculture and Rural Affairs  Guangzhou People's Republic of China"},{"name":"Department of Microbiology, Immunology &amp; Infection Diseases University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanchong Key Laboratory of Disease Prevention Control and Detection in Livestock and Poultry, Nanchong Vocational and Technical College  Nanchong People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8121965"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M211285200"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.3.1109-1120.2004"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.341"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e10-11-0874"},{"issue":"13","key":"e_1_2_10_7_1","first-page":"2798","article-title":"Membrane\u2010associated RING\u2010CH (MARCH) 8 mediates the ubiquitination and lysosomal degradation of the transferrin receptor","volume":"126","author":"Fujita H","year":"2013","journal-title":"J Cell Sci"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1205246109"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806217115"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00799-1"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2020.12.207"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01751"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.abk3067"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1393590"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1707487"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2181615"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20122695"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2004332117"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e04-03-0216"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.007435"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2016.1192752"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020105139"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-021-01520-4"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.16017"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3956"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2018.02.003"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.AC118.005907"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.03264-20"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00618-21"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01882-20"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00219-21"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01484-21"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.724403"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24724-2"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.075"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.57763"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.10.5762"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.24.12463-12472.2002"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cti2.1073"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00418-x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2164742"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06553"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15399"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature15400"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30609-9"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.2015740"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011619"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29445","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29445","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29445","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:23:34Z","timestamp":1772987014000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29445"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29445","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29445"}}