{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:45:35Z","timestamp":1790862335590,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82302527"],"award-info":[{"award-number":["82302527"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Persistent human papillomavirus (HPV) infection can lead to cervical intraepithelial neoplasia (CIN) and cervical cancer, posing serious threats to the health of women. Although the cervicovaginal microbiota is strongly associated with CIN, the dynamics of the microbiota during CIN development are unknown. In this retrospective cohort study, we analyzed 3\u2010year longitudinal data from 72 patients diagnosed with a persistent HPV infection almost all caused by high\u2010risk HPV types. Patients were categorized into groups with HPV persistent infection (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200937), progression to CIN (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200916), and CIN regression (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200919) based on infection outcome during the follow\u2010up period. Furthermore, 16S rRNA gene sequencing was performed on consecutively collected cervical samples to explore the composition and dynamics of the cervicovaginal microbiota during the development and regression of CIN. Our results showed that the composition of the cervicovaginal microbiota varied among women with different HPV infection outcomes and remained relatively stable during the follow\u2010up period. Notably, the serial follow\u2010up data showed that these microbial alterations were present for at least 1\u20132 years and occurred before pathologic changes. In addition, microbial markers that were highly discriminatory for CIN progression or regression were identified. This study provides evidence for a temporal relationship between changes in the cervicovaginal microbiota and the development of CIN, and our findings provide support for future microbial intervention strategies for CIN.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29451","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T04:44:33Z","timestamp":1706849073000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Cervicovaginal microbiota long\u2010term dynamics and prediction of different outcomes in persistent human papillomavirus infection"],"prefix":"10.1002","volume":"96","author":[{"given":"Ningqing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuyi","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirong","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Affiliated Hospital of Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Lu","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jialin","family":"Xiang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianru","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Peng","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niwei","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7118-2177","authenticated-orcid":false,"given":"Jian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University  Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University  Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2016.86"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32470-X"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pcl.2004.10.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/516784"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djq356"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e31818c9222"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soncn.2019.02.003"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.k499"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-022-01083-2"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000001343"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28595"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00143-21"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-06731-5"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1471"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477556"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-12-1406"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-023-01589-9"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt1209"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0548-6"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1070"},{"issue":"1","key":"e_1_2_9_25_1","first-page":"4680","article-title":"Vaginal microbiome of reproductive\u2010age women","volume":"108","author":"Ravel J","year":"2010","journal-title":"Proc Nat Acad Sci"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.857299"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2018.06.036"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu330"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13153664"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.129"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.918190"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008376"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-021-03201-w"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0127444"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15856-y"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14194909"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.21037\/tcr-22-2026"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006700"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-315084"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2023.2201159"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010222"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.722639"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0238705"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-022-01317-9"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28724-8"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00374"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29451","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29451","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29451","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:25:39Z","timestamp":1772987139000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29451"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29451","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-11-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-23","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29451"}}