{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T10:44:45Z","timestamp":1779187485770,"version":"3.51.4"},"reference-count":12,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T00:00:00Z","timestamp":1707264000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The positive clinical threshold of human papillomavirus\u00a0(HPV) tests validated for primary cervical cancer screening (CCS) is designed to offer an optimal balance between clinical sensitivity and specificity. However, there may be a gap between the analytical sensitivity of the test and the positive clinical threshold, referred to here as the \u201cgray\u2010zone.\u201d\u00a0This study aims to determine the prevalence and significance of HPV results obtained in the gray\u2010zone in routine practice. Cervical samples obtained in our institution for CCS over a 22\u2010month\u2010period were tested with the Alinity m HR\u2010HPV Assay (Abbott). Clinical and biological data, including cytological results and patients' HPV history were collected. Of the 6101 samples collected, 1.7% had an HPV result in the gray\u2010zone (102 patients). The proportion of gray\u2010zone results varied according to HPV genotype, reaching 11.8% of samples with detectable HPV DNA in the case of HPV31\/33\/52\/58 genotypes. Reflex cytologies showed no abnormalities or Atypical\u00a0Squamous Cells of Undetermined Significance results in 74.6% and 17.9% of cases, respectively. A previous or subsequent HPV\u2010positive result with a (possibly) identical genotype was observed in 58% and 38% of cases, respectively. Two women with a history of persistent HPV detection had a CIN2+ lesion 1 year after the gray\u2010zone result. In conclusion, the proportion of HPV results in the gray\u2010zone varies according to genotype. No cytological abnormality is observed in the majority of cases, but a few rare patients with a history of persistent HPV infection should be closely monitored even if the HPV result is transiently located in the gray\u2010zone.<\/jats:p>","DOI":"10.1002\/jmv.29465","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:48:59Z","timestamp":1707288539000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Prevalence and significance of HPV DNA detection below the clinical threshold of the commercial kit Alinity m HR\u2010HPV assay (Abbott)"],"prefix":"10.1002","volume":"96","author":[{"given":"Juliette","family":"Node","sequence":"first","affiliation":[{"name":"Laboratoire de virologie CHU de Besan\u00e7on  Besan\u00e7on France"},{"name":"CNR Papillomavirus CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanny","family":"Dor","sequence":"additional","affiliation":[{"name":"Laboratoire de biologie cellulaire CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Line","family":"Puget","sequence":"additional","affiliation":[{"name":"Laboratoire de virologie CHU de Besan\u00e7on  Besan\u00e7on France"},{"name":"CNR Papillomavirus CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajeev","family":"Ramanah","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie\u2010Obst\u00e9trique CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Cot","sequence":"additional","affiliation":[{"name":"Service de Gyn\u00e9cologie\u2010Obst\u00e9trique CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Bibeau","sequence":"additional","affiliation":[{"name":"Laboratoire d'anatomie Pathologique CHU Besan\u00e7on  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nolwen","family":"Di Domizio","sequence":"additional","affiliation":[{"name":"EA3181, UBFC LabEx LipSTIC ANR\u201011\u2010LABX\u20100021  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8430-214X","authenticated-orcid":false,"given":"Jean\u2010Luc","family":"Pr\u00e9tet","sequence":"additional","affiliation":[{"name":"CNR Papillomavirus CHU Besan\u00e7on  Besancon France"},{"name":"Laboratoire de biologie cellulaire CHU Besan\u00e7on  Besancon France"},{"name":"EA3181, UBFC LabEx LipSTIC ANR\u201011\u2010LABX\u20100021  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2892-4216","authenticated-orcid":false,"given":"Quentin","family":"Lepiller","sequence":"additional","affiliation":[{"name":"Laboratoire de virologie CHU de Besan\u00e7on  Besan\u00e7on France"},{"name":"CNR Papillomavirus CHU Besan\u00e7on  Besancon France"},{"name":"EA3181, UBFC LabEx LipSTIC ANR\u201011\u2010LABX\u20100021  Besancon France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.30716"},{"key":"e_1_2_9_3_1","article-title":"Cytology versus HPV testing for cervical cancer screening in the general population","volume":"8","author":"Koliopoulos G","year":"2017","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2020.12.061"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2021.04.031"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24010"},{"key":"e_1_2_9_7_1","unstructured":"Haute Autorit\u00e9 de Sant\u00e9 HAS. \u00c9valuation de la recherche des papillomavirus humains (HPV) en d\u00e9pistage primaire des l\u00e9sions pr\u00e9canc\u00e9reuses et canc\u00e9reuses du col de l'ut\u00e9rus et de la place du double immuno\u2010marquage p16\/Ki67. 2019. Accessed January 11 2024.https:\/\/www.has-sante.fr\/jcms\/c_2806160\/fr\/evaluation-de-la-recherche-des-papillomavirus-humains-hpv-en-depistage-primaire-des-lesions-precancereuses-et-cancereuses-du-col-de-l-uterus-et-de-la-place-du-double-immuno-marquage-p16\/ki67"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.07.023"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pvr.2015.06.006"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2020.100293"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.31883"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13164149"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27485"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29465","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29465","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29465","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:25:00Z","timestamp":1772987100000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29465"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":12,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29465"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29465","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-10-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29465"}}