{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:16:04Z","timestamp":1792181764077,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T00:00:00Z","timestamp":1709078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Cervical human papillomavirus (HPV) infection is believed to increase the risks of pregnancy failure and abortion, however, whether the uterine cavity HPV infection reduces pregnancy rate or increases miscarriage rate remains unclarified in infertile women undergoing assisted reproductive technology (ART) treatment. Therefore, we aimed to assess ART outcomes in the presence of intrauterine HPV.\u00a0This was a hospital\u2010based multicenter (five\u00a0reproductive medicine centers) matched cohort study. This study involved 4153 infertile women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection\u00a0treatment in five reproductive medicine centers between October 2018 and 2020. The spent embryo transfer media sample with endometrium tissue were collected and performed with flow\u2010through hybridization and gene chips to detect HPV DNA. According to basic characteristics, HPV\u2010positive and negative patients were matched in a ratio of 1:4 by age, body mass index\u00a0transfer timing, transfer type, and number of embryos transferred. The primary outcome was pregnancy and clinical miscarriage rates in the transfer cycle underwent HPV detection.\u00a092\u00a0HPV\u2010positive and 368 HPV\u2010negative patients were screened and analyzed statistically. Univariate analysis showed uterine cavity HPV infection resulted in lower rates of ongoing pregnancy (31.5% vs. 44.6%; <jats:italic>p\u2009<\/jats:italic>=\u20090.023), implantation (32.3% vs. 43.1%; <jats:italic>p\u2009<\/jats:italic>=\u20090.026), biochemical pregnancy (47.8% vs. 62.5%; <jats:italic>p\u2009<\/jats:italic>=\u20090.010), and clinical pregnancy (40.2% vs. 54.3%; <jats:italic>p\u2009<\/jats:italic>=\u20090.015) compared with HPV negative group. The infertile female with positive HPV also had a slightly higher frequency of biochemical miscarriage (15.9% vs. 13.0%; <jats:italic>p\u2009<\/jats:italic>=\u20090.610) and clinical miscarriage (24.3% vs. 15.5%; <jats:italic>p\u2009<\/jats:italic>=\u20090.188).\u00a0These findings suggest that HPV infection in the uterine cavity is a high risk for ART failure. HPV screening is recommended before ART treatment, which may be benefit to improving pregnancy outcome.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jmv.29468","type":"journal-article","created":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T05:34:56Z","timestamp":1709098496000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Assisted reproductive technology treatment failure and the detection of intrauterine HPV through spent embryo transfer media sample"],"prefix":"10.1002","volume":"96","author":[{"given":"Mingzhu","family":"Bai","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, Department of Obstertrics and Gynecology, Tongji Hospital, School of Medicine Tongji University Shanghai China"},{"name":"Center for Reproductive Medicine, Maternal and Child Health Hospital in Xuzhou Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6010-7331","authenticated-orcid":false,"given":"Di","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Reproductive Medicine Center,\u00a0Shanghai General Hospital Shanghai Jiaotong University Shanghai China"},{"name":"School of Medicine, Center for Reproductive Medicine &amp; Fertility Preservation Program, International Peace Maternity and Child Health Hospital Shanghai Jiao Tong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhui","family":"Shu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Maternal and Child Health Hospital in Guangxi Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wei","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Liuzhou Worker's Hospital Liuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Maternal and Child Health Hospital in Xuzhou Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenya","family":"Yuan","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Maternal and Child Health Hospital in Xuzhou Xuzhou Medical University Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suying","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine, Zhongshan Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Kang","sequence":"additional","affiliation":[{"name":"Center of Clinical Research, Shanghai General Hospital Shanghai Jiaotong University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zikai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Department of Obstertrics and Gynecology, Tongji Hospital, School of Medicine Tongji University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shanghai Wusong Hospital Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Obstetrics and Gynecology University of Texas Southwestern Medical Center Dallas Texas USA"},{"name":"Harold C. 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