{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:53:12Z","timestamp":1786755192258,"version":"build-2736575974"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T00:00:00Z","timestamp":1708387200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The mpox outbreak has subdued with fewer reported cases at the present in high\u2010income countries. It is known that mpox virus (MPXV) infection has been epidemic for more than 50 years in African countries. The ancestral MPXV strain has changed into multiple clades, indicating the ongoing evolution of MPXV, which reflects the historical neglect of mpox in Africa, especially after smallpox eradication, and bestows the danger of more severe mpox epidemics in the future. It is thus imperative to continue the development of mpox diagnostics and treatments so we can be prepared in the event of a new mpox epidemic. In this study, we have developed an MPXV detection tool that leverages the recombinase\u2010aid amplification assay by integrating lateral flow strips (RAA\u2010LF) and one\u2010step sample DNA preparation, with visible readout, no need of laboratory instrument, and ready for field deployment. The detection limit reaches 10 copies per reaction. The performance of our RAA\u2010FL assay in diagnosing mpox clinical samples is on par with that of the quantitative polymerase chain reaction (PCR) assay. Taken together, we have developed a point\u2010of\u2010care RAA\u2010LF method of high accuracy and sensitivity, readily deployable for field detection of MPXV. This diagnostic tool is expected to improve and accelerate field\u2010 and self\u2010diagnosis, allow timely isolation and treatment, reduce the spread of MPXV, thus effectively mitigate MPXV outbreak in the future.<\/jats:p>","DOI":"10.1002\/jmv.29469","type":"journal-article","created":{"date-parts":[[2024,2,20]],"date-time":"2024-02-20T11:57:30Z","timestamp":1708430250000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["A field diagnostic method for rapid and sensitive detection of mpox virus"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9015-2646","authenticated-orcid":false,"given":"Fei","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1003-5453","authenticated-orcid":false,"given":"Fengwen","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Wang","sequence":"additional","affiliation":[{"name":"Christophe M\u00e9rieux Laboratory, National Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Song","sequence":"additional","affiliation":[{"name":"Beijing Ditan Hospital Capital Medical University Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yamei","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Wei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Xie","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Mei","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingwa","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhao","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Guo","sequence":"additional","affiliation":[{"name":"Christophe M\u00e9rieux Laboratory, National Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jugao","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Head and Neck Surgery Capital Medical University  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Ren","sequence":"additional","affiliation":[{"name":"Christophe M\u00e9rieux Laboratory, National Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8496-172X","authenticated-orcid":false,"given":"Ronghua","family":"Jin","sequence":"additional","affiliation":[{"name":"Beijing Ditan Hospital Capital Medical University Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1116-4559","authenticated-orcid":false,"given":"Jianwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Christophe M\u00e9rieux Laboratory, National Institute of Pathogen Biology Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Guo","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pathogen Infection Prevention and Control (Ministry of Education) State Key Laboratory of Respiratory Health and Multimorbidity  Beijing China"},{"name":"NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology and Center for AIDS Research Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105794"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.05.030"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-022-01907-y"},{"key":"e_1_2_9_5_1","unstructured":"Monkeypox: experts give virus variants new names.Saudi Med J.2022;43(9):1069\u20101070."},{"key":"e_1_2_9_6_1","unstructured":"World Health Organization. 2022\u201323 mpox (monkeypox) outbreak: global trends. 2023. Accessed May 9 2023.https:\/\/worldhealthorg.shinyapps.io\/mpx_global\/"},{"issue":"38","key":"e_1_2_9_7_1","first-page":"853","article-title":"The first imported case of monkeypox in the mainland of China\u2014Chongqing municipality, China, September 16, 2022","volume":"4","author":"Zhao H","year":"2022","journal-title":"China CDC Wkly"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-072410"},{"issue":"3","key":"e_1_2_9_9_1","first-page":"269","article-title":"Update on mpox: a brief narrative review","volume":"31","author":"Antinori S","year":"2023","journal-title":"Infez Med"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2023.28.27.2200806"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(22)00574-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41563-020-00906-z"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmaid.2022.102459"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2022.105373"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00256-7"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-023-01903-5"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.3c05007"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202105231"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40820-022-00888-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwac104"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crmeth.2023.100620"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(23)00148-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2015.1090877"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.202200242"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-023-05848-w"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-81376-4"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics13071349"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2022.117009"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010141"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.202300455"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11102513"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiad406"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1137968"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0112146"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29469","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jmv.29469","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29469","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:24:49Z","timestamp":1772987089000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29469"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1002\/jmv.29469"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29469","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2]]},"assertion":[{"value":"2023-12-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29469"}}