{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:47:58Z","timestamp":1788997678005,"version":"build-2803163510"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T00:00:00Z","timestamp":1708300800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>This study aimed to analyze the human papillomavirus (HPV) genotype distribution in a large cohort of high\u2010grade vaginal intraepithelial neoplasia (VaIN) (vaginal HSIL, VaIN2\/3) patients from two Italian referral centers. We included all patients with histologically confirmed VaIN2\/3 from the Department of Surgical Sciences, Sant'Anna Hospital, University of Torino, Torino, Italy, and Ospedale Maggiore della Carit\u00e0, Novara, Italy, between 2003 and 2022. After the histological evaluation of formalin\u2010fixed paraffin\u2010embedded samples, we performed HPV genotyping with VisionArray HPV Chip 1.0. We detected HPV DNA in 94.4% of VaIN2\/3 (168\/178), with HPV 16 as the most prevalent genotype, accounting for 51.8% of all infections, 41.2% of VaIN2\u00a0and 77.6% of VaIN3 cases. Other frequent genotypes were HPV 58 (8.3%, 10.9% of VaIN2\u00a0and 2.0% of VaIN3), HPV 73 (5.4%, 5.0% of VaIN2\u00a0and 6.1% of VaIN3), and HPV 31 (5.4%, 6.7% of VaIN2\u00a0and 2.0% of VaIN3). 73.2% of VaIN2\/3 had a single HPV genotype infection and 26.8% a multiple infection (20.8% a double infection, 4.8% a triple infection, and 1.2% a quadruple infection). Single infection was more frequently present in VaIN3 than VaIN2 (81.6% vs. 69.8%). 69.1% of single infections and 73.3% of multiple infections had one or more genotypes covered by nine\u2010valent HPV vaccine. HPV vaccination is expected to have a large impact on reducing the incidence of vaginal intraepithelial neoplasia.<\/jats:p>","DOI":"10.1002\/jmv.29474","type":"journal-article","created":{"date-parts":[[2024,2,19]],"date-time":"2024-02-19T13:44:00Z","timestamp":1708350240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Human papillomavirus genotyping in high\u2010grade vaginal intraepithelial neoplasia: A\u00a0multicentric Italian study"],"prefix":"10.1002","volume":"96","author":[{"given":"Mario","family":"Preti","sequence":"first","affiliation":[{"name":"Obstetrics and Gynecology Unit 1, Department of Surgical Sciences, Sant' Anna\u00a0Hospital University of Torino Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renzo","family":"Boldorini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3041-1201","authenticated-orcid":false,"given":"Niccol\u00f2","family":"Gallio","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Unit 2, Department of Surgical Sciences, Sant' Anna Hospital University of Torino Torino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Cavagnetto","sequence":"additional","affiliation":[{"name":"Department of Maternal\u2010Neonatal and Infant Health, Division of Obstetrics and Gynaecology, Ospedale Degli Infermi University of Turin Biella Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8398-7557","authenticated-orcid":false,"given":"Fulvio","family":"Borella","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Unit 1, Department of Surgical Sciences, Sant' Anna\u00a0Hospital University of Torino Torino 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