{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T04:41:52Z","timestamp":1759380112558,"version":"build-2065373602"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T00:00:00Z","timestamp":1709164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003786","name":"Hangzhou Science and Technology Bureau","doi-asserted-by":"publisher","award":["J\u2010202106"],"award-info":[{"award-number":["J\u2010202106"]}],"id":[{"id":"10.13039\/501100003786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infection with influenza A virus (IAV) and severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) poses a significant risk to human life, health, and the global economy. Vaccination is one of the most effective strategies in the fight against infectious viruses. In this study, we, for the first time, have evaluated the immunogenicity and protective effect of an influenza\/SARS\u2010CoV\u20102 Omicron subunit combined vaccine adjuvanted with MF59 and administered to BALB\/c mice. Results showed that the combined vaccine induced high levels of IgG, IgG<jats:sub>1<\/jats:sub>, and IgG<jats:sub>2a<\/jats:sub> antibodies, as well as influenza A H1N1\/California\/2009 virus\u2010specific hemagglutination\u2010inhibiting antibodies in BALB\/c mice. Moreover, this subunit combined vaccine induced high titers of neutralization antibodies against SARS\u2010CoV\u20102 Omicron sublineage BA.5 pseudovirus and effectively reduced the viral load of authentic SARS\u2010CoV\u20102 Omicron sublineage BA.5.2 in the cell culture supernatants. These results suggested that this subunit combined vaccine achieved protective effect against both H1N1 A\/California\/07\/2009 strain and SARS\u2010CoV\u20102 Omicron BA.5.2 variant. It is therefore expected that this study will establish the scientific foundation for the next\u2010step development of combined vaccines against other strains or variants of IAV and SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/jmv.29479","type":"journal-article","created":{"date-parts":[[2024,3,1]],"date-time":"2024-03-01T02:58:03Z","timestamp":1709261883000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["A subunit\u2010based influenza\/SARS\u2010CoV\u20102 Omicron combined vaccine induced potent protective immunity in BALB\/c mice"],"prefix":"10.1002","volume":"96","author":[{"given":"Naru","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zihui","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC\/CAMS), Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luying","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuewen","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuchang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rouhan","family":"Ye","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyin","family":"Dong","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8283-7135","authenticated-orcid":false,"given":"Shibo","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Molecular Virology (MOE\/NHC\/CAMS), Shanghai Frontiers Science Center of Pathogenic Microbes and Infection, School of Basic Medical Sciences, Shanghai Institute of Infectious Disease and Biosecurity Fudan University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haijun","family":"Han","sequence":"additional","affiliation":[{"name":"School of Medicine Hangzhou City University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,29]]},"reference":[{"issue":"3","key":"e_1_2_10_2_1","first-page":"162","article-title":"SARS\u2010CoV\u20102 variant biology: immune escape, transmission and fitness","volume":"21","author":"Carabelli AM","year":"2023","journal-title":"Nat Rev Microbiol"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2381"},{"key":"e_1_2_10_4_1","unstructured":"World Health Organization. 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