{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:16:10Z","timestamp":1790154970949,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T00:00:00Z","timestamp":1710374400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272436"],"award-info":[{"award-number":["32272436"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human norovirus (HuNoV) is the most predominant viral agents of acute gastroenteritis. Point\u2010of\u2010care testing (POCT) based on lateral flow immunochromatography (LIFC) has become an important tool for rapid diagnosis of HuNoVs. However, low sensitivity and lack of quantitation are the bottlenecks of traditional LIFC. Thus, we established a rapid and accurate technique that combined immunomagnetic enrichment (IM) with LFIC to identify GII HuNoVs in fecal specimens. Before preparing immunofluorescent nanomagnetic microspheres and achieving the effect of HuNoV enrichment in IM and fluorescent signal in LFIC, amino\u2010functionalized magnetic beads (MBs) and carboxylated quantum dots (QDs) were coupled at a mass ratio of 4:10. Anti\u2010HuNoV monoclonal antibody was then conjugated with QDs\u2010MB. The limit of detection was 1.56\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009copies\/mL, and the quantitative detection range was 1.56\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009copies\/mL\u20131\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    \u2009copies\/mL under optimal circumstances. The common HuNoV genotypes GII.2, GII.3, GII.4, and GII.17 can be detected, there was no cross\u2010reaction with various enteric viruses, including rotavirus, astrovirus, enterovirus, and sapovirus. A comparison between IM\u2010LFIC and RT\u2010qPCR for the detection of 87 fecal specimens showed a high level of agreement (kappa\u2009=\u20090.799). This suggested that the method is rapid and sensitive, making it a promising option for point\u2010of\u2010care testing in the future.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29487","type":"journal-article","created":{"date-parts":[[2024,3,14]],"date-time":"2024-03-14T06:01:14Z","timestamp":1710396074000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Rapid and sensitive lateral flow biosensor for the detection of GII human norovirus based on immunofluorescent nanomagnetic microspheres"],"prefix":"10.1002","volume":"96","author":[{"given":"Junshan","family":"Gao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Xue","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijing","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Microbial Safety and Health, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences National Health Commission Science and Technology Innovation Platform for Nutrition and Safety of Microbial Food Guangzhou Guangdong 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