{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:45:13Z","timestamp":1764701113202,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T00:00:00Z","timestamp":1710892800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271166","81970880"],"award-info":[{"award-number":["82271166","81970880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai Municipality","doi-asserted-by":"publisher","award":["22ZR1410100"],"award-info":[{"award-number":["22ZR1410100"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A large\u2010scale outbreak of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS\u2010CoV\u20102) occurred in Shanghai, China, in early December 2022. To study the incidence and characteristics of otitis media with effusion (OME) complicating SARS\u2010CoV\u20102, we collected 267 middle ear effusion (MEE) samples and 172 nasopharyngeal (NP) swabs from patients. The SARS\u2010CoV\u20102 virus was detected by RT\u2010PCR targeting. The SARS\u2010CoV\u20102 virus, angiotensin\u2010converting enzyme 2 (ACE2), and transmembrane serine protease 2 (TMPRSS2) expression in human samples was examined via immunofluorescence. During the COVID\u201019 epidemic in 2022, the incidence of OME (3%) significantly increased compared to the same period from 2020 to 2022. Ear symptoms in patients with SARS\u2010CoV\u20102 complicated by OME generally appeared late, even after a negative NP swab, an average of 9.33\u2009\u00b1\u20096.272 days after COVID\u201019 infection. The SARS\u2010CoV\u20102 virus was detected in MEE, which had a higher viral load than NP swabs. The insertion rate of tympanostomy tubes was not significantly higher than in OME patients in 2019\u20132022. Virus migration led to high viral loads in MEE despite negative NP swabs, indicating that OME lagged behind respiratory infections but had a favorable prognosis. Furthermore, middle ear tissue from adult humans coexpressed the ACE2 receptor for the SARS\u2010CoV\u20102 virus and the TMPRSS2 cofactors required for virus entry.<\/jats:p>","DOI":"10.1002\/jmv.29545","type":"journal-article","created":{"date-parts":[[2024,3,20]],"date-time":"2024-03-20T06:34:51Z","timestamp":1710916491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of SARS\u2010CoV\u20102 virus in middle ear effusions and its association with otitis media with effusion"],"prefix":"10.1002","volume":"96","author":[{"given":"Xiao","family":"Fu","sequence":"first","affiliation":[{"name":"Department of Otology and Skull Base Surgery EYE and ENT Hospital of Fudan University Shanghai China"},{"name":"Shanghai clinical medical center of hearing medicine Shanghai China"},{"name":"Key Laboratory of Hearing Medicine Ministry of Health Shanghai 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