{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:38Z","timestamp":1786977998719,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T00:00:00Z","timestamp":1710979200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005762","name":"National Taiwan University Hospital","doi-asserted-by":"publisher","award":["NTUH 112\u2010M0024"],"award-info":[{"award-number":["NTUH 112\u2010M0024"]}],"id":[{"id":"10.13039\/501100005762","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Neutrophil extracellular traps (NETs) play a role in innate pathogen defense and also trigger B\u2010cell response by providing antigens. NETs have been linked to vaccine\u2010induced thrombotic thrombocytopenia. We postulated a potential link between NET biomarkers, NET\u2010promoting autoantibodies, and adverse events (AEs) after COVID\u201019 vaccine boosters. Healthy donors (HDs) who received ChAdOx1\u2010S (A), mRNA\u20101273 (M), or recombinant protein (MVC\u2013COV1901) vaccines at the National Taiwan University Hospital between 2021 and 2022 were recruited. We measured serial NET\u2010associated biomarkers, citrullinated\u2010histone3 (citH3), and myeloperoxidase (MPO)\u2010DNA. Serum citH3 and MPO\u2010DNA were significantly or numerically higher in HDs who reported AEs (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009100, booster Day 0\/Day 30,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.01\/\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.03 and\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.30\/\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.35, respectively). We also observed a positive correlation between rash occurrence in online diaries and elevated citH3. A linear mixed model also revealed significantly higher citH3 levels in mRNA\u20101273\/ChAdOx1\u2010S recipients than MVC\u2010COV1901 recipients. Significant positive correlations were observed between the ratios of anti\u2010heparin platelet factor 4 and citH3 levels on Booster Day 0 and na\u00efve and between the ratios of anti\u2010NET IgM and citH3 on Booster Day 30\/Day 0 in the AA\u2010M and MM\u2010M group, respectively. The increased levels of citH3\/MPO\u2010DNA accompanied by NET\u2010promoting autoantibodies suggest a potential connection between mRNA\u20101273\/ChAdOx1\u2010S vaccines and cardiovascular complications. These findings provide insights for risk assessments of future vaccines.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29556","type":"journal-article","created":{"date-parts":[[2024,3,21]],"date-time":"2024-03-21T06:36:13Z","timestamp":1711002973000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Temporal changes in biomarkers of neutrophil extracellular traps and NET\u2010promoting autoantibodies following adenovirus\u2010vectored, mRNA, and recombinant protein COVID\u201019 vaccination"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5797-6093","authenticated-orcid":false,"given":"Yu\u2010Min","family":"Kuo","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Division of Allergy, Immunology and Rheumatology National Taiwan University Taipei Taiwan"},{"name":"Graduate Institute of Clinical Medicine, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun\u2010Min","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Yun","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2010Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infection National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1633-020X","authenticated-orcid":false,"given":"Shiang\u2010Jong","family":"Tzeng","sequence":"additional","affiliation":[{"name":"Department and Graduate Institute of Pharmacology, College of Medicine National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Chieh","family":"Hsu","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Infection National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5855-6170","authenticated-orcid":false,"given":"Shey\u2010Ying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, National Taiwan University Hospital National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song\u2010Chou","family":"Hsieh","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Division of Allergy, Immunology and Rheumatology National Taiwan University Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010San","family":"Chia","sequence":"additional","affiliation":[{"name":"Department of Dentistry, National Taiwan University Hospital National Taiwan University College of Medicine  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7824-3454","authenticated-orcid":false,"given":"Chiau\u2010Jing","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine Taipei Medical University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7502-9225","authenticated-orcid":false,"given":"Po\u2010Ren","family":"Hsueh","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine and Internal Medicine, China Medical University Hospital China Medical University  Taichung Taiwan"},{"name":"Departments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital National Taiwan University  Taipei Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,3,21]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1092385"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.114.011432"},{"issue":"11","key":"e_1_2_10_4_1","article-title":"Neutrophil extracellular traps in COVID\u201019","volume":"5","author":"Zuo Y","year":"2020","journal-title":"JCI Insight"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abd3876"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00536-9"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa756"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201811-2111OC"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.68563"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep37252"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.150862"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1414055112"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32946-1"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12959-021-00318-2"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.1155727"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2021.279289"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2021.102662"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0000000000000782"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2022.02.002"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.41460"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(21)00402-1"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-12750-z"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7774\/cevr.2022.11.1.96"},{"issue":"4","key":"e_1_2_10_24_1","first-page":"1493","article-title":"Durability and immunogenicity of neutralizing antibodies response against omicron variants after three doses of subunit SARS\u2010CoV\u20102 vaccine MVC\u2010COV1901: an extension to an open\u2010label, dose\u2010escalation phase 1 study","volume":"11","author":"Hsieh SM","year":"2022","journal-title":"Infect Dis Ther"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.5COVR0621-320RR"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aax2398"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.181058"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021013231"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31013-4"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/15384047.2019.1598759"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-022-06180-3"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.39938"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.42489"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-11623-9"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2105290"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12959-023-00533-z"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.04.001"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.03.008"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfma.2023.04.017"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.09.002"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.08.006"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2023.08.002"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmii.2022.09.004"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048111"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044606"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminhematol.2022.03.002"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19124090"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.018993"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2018.08.010"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.14924"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.708302"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120389"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29556","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T04:25:35Z","timestamp":1759379135000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3]]},"references-count":51,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1002\/jmv.29556"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29556","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3]]},"assertion":[{"value":"2023-11-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29556"}}