{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T03:44:57Z","timestamp":1785296697146,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T00:00:00Z","timestamp":1712016000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Persistent infection with high\u2010risk human papillomavirus (HR\u2010HPV) is a well\u2010established risk factor to the development of cervical intraepithelial neoplasia (CIN), a condition that can progress to cervical cancer (CC) a major health problem worldwide. Recently, there has been growing interest in exploring alternative therapies utilizing natural products, among which is the algae species <jats:italic>Laurencia johnstonii<\/jats:italic> Setchell &amp; Gardner, 1924 (<jats:italic>L. johnstonii<\/jats:italic>), proposed for the management of precancerous lesions. The aim of this work was to determine the effect of an organic extract from <jats:italic>L. johnstonii<\/jats:italic> (ELj) in early cervical lesions (CIN 1). These CIN 1 lesions were generated in a murine model expressing the HR\u2010HPV16 E7 oncoprotein (K14E7HPV transgenic mice) with a single exogenous hormonal stimulus using 17\u03b2\u2010estradiol. The histopathological studies, the determination of cell proliferation and of the apoptotic levels in cervical tissue, showed that, seven doses of ELj (30\u2009mg\/kg weight per day diluted in a DMSO\u2010saline solution [1:7]) lead to recovery the architecture of cervical epithelium. Accordingly, in the transgenic mice it was observed a statistically significant decrease of the PCNA expression levels, a marker of cell proliferation, and a statistically significant increase in the apoptosis levels using Caspase 3 as a marker. In addition, we determined the expression levels of the tumor suppressor miR\u2010218 and the oncomiRNA miR\u201021. Interestingly, our results may suggest that ELj treatment tended to restore the normal expression of both miRNAs as compared with controls being more evident in the non\u2010transgenic induced mice. Differences of <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05 were considered statistically significant through the whole study. Based on these results, we propose that the use of ELj could be an alternative for the treatment of cervical early lesions.<\/jats:p>","DOI":"10.1002\/jmv.29571","type":"journal-article","created":{"date-parts":[[2024,4,2]],"date-time":"2024-04-02T14:04:19Z","timestamp":1712066659000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Laurencia johnstonii<\/i> extract reverses early lesions in the K14E7HPV16 murine cervical carcinogenesis model"],"prefix":"10.1002","volume":"96","author":[{"given":"Erandi","family":"Arvizu\u2010Hernandez","sequence":"first","affiliation":[{"name":"Laboratory of Cellular Biology and Natural Products I, Escuela Nacional de Medicina y Homeopat\u00eda Instituto Polit\u00e9cnico Nacional  La Paz Mexico"},{"name":"Department of Genetics and Molecular Biology Centro de Investigaci\u00f3n y de Estudios Avanzados IPN  Ciudad de Mexico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Judith","family":"Hernandez\u2010Guerrero","sequence":"additional","affiliation":[{"name":"Department of Technologies Development, Centro Interdisciplinario de Ciencias Marinas Instituto Polit\u00e9cnico Nacional  La Paz B.C.S. Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Alvarez\u2010Rios","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro de Investigaci\u00f3n y de Estudios Avanzados IPN  Ciudad de Mexico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0024-7076","authenticated-orcid":false,"given":"Patricio","family":"Gariglio","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro de Investigaci\u00f3n y de Estudios Avanzados IPN  Ciudad de Mexico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Cornejo\u2010Garrido","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular Biology and Natural Products I, Escuela Nacional de Medicina y Homeopat\u00eda Instituto Polit\u00e9cnico Nacional  La Paz Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4576-881X","authenticated-orcid":false,"given":"Rodolfo","family":"Ocadiz\u2010Delgado","sequence":"additional","affiliation":[{"name":"Department of Genetics and Molecular Biology Centro de Investigaci\u00f3n y de Estudios Avanzados IPN  Ciudad de Mexico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)32470-X"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.03116"},{"key":"e_1_2_10_4_1","first-page":"4862","article-title":"Dissection of human papillomavirus E6 and E7 function in transgenic mouse models of cervical carcinogenesis","volume":"63","author":"Riley RR","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13105-021-00818-9"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.13093"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4590-4"},{"issue":"201","key":"e_1_2_10_8_1","first-page":"1","article-title":"Antitumoral effect of Lauritenol on 3D culture of breast cancer explants","volume":"17","author":"Garc\u00eda\u2010Davis S","year":"2019","journal-title":"Mar Drugs"},{"issue":"29","key":"e_1_2_10_9_1","first-page":"695","article-title":"XXIX expedition of the california academy of sciences to the Gulf of California in 1921. 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