{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T16:36:53Z","timestamp":1770741413006,"version":"3.49.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The lower respiratory system serves as the target and barrier for beta\u2010coronavirus (beta\u2010CoV) infections. In this study, we explored beta\u2010CoV infection dynamics in human bronchial epithelial (HBE) organoids, focusing on HCoV\u2010OC43, SARS\u2010CoV, MERS\u2010CoV, and SARS\u2010CoV\u20102. Utilizing advanced organoid culture techniques, we observed robust replication for all beta\u2010CoVs, particularly noting that SARS\u2010CoV\u20102 reached peak viral RNA levels at 72\u2009h postinfection. Through comprehensive transcriptomic analysis, we identified significant shifts in cell population dynamics, marked by an increase in goblet cells and a concurrent decrease in ciliated cells. Furthermore, our cell tropism analysis unveiled distinct preferences in viral targeting: HCoV\u2010OC43 predominantly infected club cells, while SARS\u2010CoV had a dual tropism for goblet and ciliated cells. In contrast, SARS\u2010CoV\u20102 primarily infected ciliated cells, and MERS\u2010CoV showed a marked affinity for goblet cells. Host factor analysis revealed the upregulation of genes encoding viral receptors and proteases. Notably, HCoV\u2010OC43 induced the unfolded protein response pathway, which may facilitate viral replication. Our study also reveals a complex interplay between inflammatory pathways and the suppression of interferon responses during beta\u2010CoV infections. These findings provide insights into host\u2010virus interactions and antiviral defense mechanisms, contributing to our understanding of beta\u2010CoV infections in the respiratory tract.<\/jats:p>","DOI":"10.1002\/jmv.29600","type":"journal-article","created":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T09:16:07Z","timestamp":1712654167000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Differential beta\u2010coronavirus infection dynamics in human bronchial epithelial organoids"],"prefix":"10.1002","volume":"96","author":[{"given":"Dongbin","family":"Park","sequence":"first","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Se\u2010Mi","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hobin","family":"Jang","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanghee","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"},{"name":"College of Medicine and Medical Research Institute Chungbuk National University  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho young","family":"Ji","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heedong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute Chungbuk National University  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woohyun","family":"Kwon","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"},{"name":"College of Medicine and Medical Research Institute Chungbuk National University  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yeonglim","family":"Kang","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhee","family":"Hwang","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunjoon","family":"Kim","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark Anthony B.","family":"Casel","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"},{"name":"College of Medicine and Medical Research Institute Chungbuk National University  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Issac","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeong\u2010Sun","family":"Yang","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Center for Laboratory Control of Infectious Disease Korea National Institute of Health (KNIH)  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joo\u2010Yeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Center for Laboratory Control of Infectious Disease Korea National Institute of Health (KNIH)  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ki","family":"Choi","sequence":"additional","affiliation":[{"name":"Center for Study of Emerging and Re\u2010emerging Viruses, Korea Virus Research Institute Institute for Basic Science (IBS)  Daejeon Republic of Korea"},{"name":"College of Medicine and Medical Research Institute Chungbuk National University  Cheongju Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14630-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30185-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2282"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2021.628370"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28231"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26362"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30211-7"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2020.03.023"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-020-0259-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2901-9"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb7314"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.030"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.02.085"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ1020"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.13721"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113077"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giaa151"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2019.03.003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-015-0844-5"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-128"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0969-x"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-27717-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2119680119"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2218083120"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03569-1"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvac139"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-00630-3"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.04.003"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3001143"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-00958-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108175"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00426-x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02232-10"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01890-13"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.02.052"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2021.10.016"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.01.053"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.608645"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2022.198897"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11020253"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06990-9"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.10.004"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00431-18"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s43556-022-00090-3"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.107118"},{"key":"e_1_2_9_49_1","article-title":"The SARS\u2010CoV\u20102 envelope (E) protein forms a calcium\u2010 and voltage\u2010activated calcium channel","author":"Antonides LH","year":"2022","journal-title":"bioRxiv"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03610-3"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.29309"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12968-y"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.06.070"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25551-1"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29600","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T19:15:45Z","timestamp":1744917345000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jmv.29600"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29600","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29600"}}