{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:58:24Z","timestamp":1753869504873,"version":"3.41.2"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Interferon lambda (IFN\u03bb), classified as a type III IFN, is a representative cytokine that plays an important role in innate immunity along with type I IFN. IFN\u03bb can elicit antiviral states by inducing peculiar sets of IFN\u2010stimulated genes (ISGs). In this study, an adenoviral vector expression system with a tetracycline operator system was used to express human IFN\u03bb4 in cells and mice. The formation of recombinant adenovirus (rAd\u2010huIFN\u03bb4) was confirmed using immunohistochemistry assays and transmission electron microscopy. Its purity was verified by quantifying host cell DNA and host cell proteins, as well as by confirming the absence of the replication\u2010competent adenovirus. The transduction of rAd\u2010huIFN\u03bb4 induced ISGs and inhibited four subtypes of the influenza virus in both mouse\u2010derived (LA\u20104) and human\u2010derived cells (A549). The antiviral state was confirmed in BALB\/c mice following intranasal inoculation with 10<jats:sup>9<\/jats:sup> PFU of rAd\u2010huIFN\u03bb4, which led to the inhibition of four subtypes of the influenza virus in mouse lungs, with reduced inflammatory lesions. These results imply that human IFN\u03bb4 could induce antiviral status by modulating ISG expression in mice.<\/jats:p>","DOI":"10.1002\/jmv.29605","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T10:48:36Z","timestamp":1713437316000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antiviral activity of adenoviral vector expressing human interferon lambda\u20104 against influenza virus"],"prefix":"10.1002","volume":"96","author":[{"given":"Dong\u2010Hwi","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae\u2010Hyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyu\u2010Beom","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joong\u2010Bok","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seung\u2010Yong","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Seon","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang\u2010Won","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong\u2010Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7266-6571","authenticated-orcid":false,"given":"In\u2010Soo","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine Konkuk University  Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"Konkuk University Zoonotic Diseases Research Center Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"},{"name":"KU Center for Animal Blood Medical Science Konkuk University Gwangjin\u2010gu Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-120617-041310"},{"volume-title":"Textbook of Influenza","year":"2014","author":"Robert GW","key":"e_1_2_10_3_1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-019-2539-x"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2018.07.001"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-104"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/315915"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03671-z"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2007.10.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni875"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni873"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.5772\/18783"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2014.07.022"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2018.10.007"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01703-09"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2021.198342"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-022-10000-1"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2005.16.393"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.33354"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2016-0174OC"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103919"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4274\/tjps.galenos.2021.25664"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20220839"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2014.12.003"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.735576"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9120989"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30566-X"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22177-1"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2018.0066"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1590\/1414-431x20165646"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-53457-2_3"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.07.024"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc2061"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00376-12"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026072"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22328"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003548"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.02.019"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/jir.2016.0031"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01732-09"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3369"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29605","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T19:15:59Z","timestamp":1744917359000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jmv.29605"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29605","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"type":"print","value":"0146-6615"},{"type":"electronic","value":"1096-9071"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2024-01-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29605"}}