{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:21:38Z","timestamp":1775640098248,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T00:00:00Z","timestamp":1713744000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vertical transmission has been described following monkeypox virus (MPXV) infection in pregnant women. The presence of MPXV has been reported in the placenta from infected women, but whether pathogens colonize placenta remains unexplored. We identify trophoblasts as a target cell for MPXV replication. In a pan\u2010microscopy approach, we decipher the specific infectious cycle of MPXV and inner cellular structures in trophoblasts. We identified the formation of a specialized region for viral morphogenesis and replication in placental cells. We also reported infection\u2010induced cellular remodeling. We found that MPXV stimulates cytoskeleton reorganization with intercellular extensions for MPXV cell spreading specifically to trophoblastic cells. Altogether, the specific infectious cycle of MPXV in trophoblast cells and these protrusions that were structurally and morphologically similar to filopodia reveal new insights into the infection of MPXV.<\/jats:p>","DOI":"10.1002\/jmv.29620","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T11:00:51Z","timestamp":1713783651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pan\u2010microscopic examination of monkeypox virus in trophoblasts cells reveals new insights into virions release through filopodia\u2010like projections"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3603-0426","authenticated-orcid":false,"given":"Jonatane","family":"Andrieu","sequence":"first","affiliation":[{"name":"Centre National de la Recherche Scientifique, \u00c9tablissement Fran\u00e7ais du Sang, Anthropologie bio\u2010culturelle, Droit,\u00a0\u00c9thique et Sant\u00e9 Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaux","family":"Valade","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Lebideau","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florence","family":"Bretelle","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"},{"name":"D\u00e9partement de gyn\u00e9cologie et d'obst\u00e9trique, Gyn\u00e9pole La Conception, AP\u2010HM Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Louis","family":"M\u00e8ge","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, \u00c9tablissement Fran\u00e7ais du Sang, Anthropologie bio\u2010culturelle, Droit,\u00a0\u00c9thique et Sant\u00e9 Aix\u2010Marseille University Marseille France"},{"name":"Laboratoire d'Immunologie Assistance Publique H\u00f4pitaux de Marseille (APHM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"Wurtz","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2285-7051","authenticated-orcid":false,"given":"Soraya","family":"Mezouar","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique, \u00c9tablissement Fran\u00e7ais du Sang, Anthropologie bio\u2010culturelle, Droit,\u00a0\u00c9thique et Sant\u00e9 Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8006-7704","authenticated-orcid":false,"given":"Bernard","family":"La Scola","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"Baudoin","sequence":"additional","affiliation":[{"name":"Institut Recherche D\u00e9veloppement, Assistance Publique\u2014H\u00f4pitaux de Marseille, Microbe, Evolution, Phylogeny Infection Aix\u2010Marseille University Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00775-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28560"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.19265"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2022.12.027"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2207323"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2022.07.005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.36.2200620"},{"key":"e_1_2_9_9_1","unstructured":"BrownB FrickeI ImarogbeC et al. Virus monkeypox et aper\u00e7u de ses prot\u00e9ines immunomodulatrices\u2014Weaver\u20142008\u2014Revues immunologiques.Explor immunol. 2023. Wiley Online Library [Internet]. [cited June 21 Wiley Online Library [Internet]. [cited June 21 http:\/\/onlinelibrary.wiley.com\/doi\/full\/10.1111\/j.1600-065X.2008.00691.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1959.tb00328.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v15081748"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11071781"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28878"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01969-9"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28536"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200412169"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gofs.2023.03.002"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1304718110"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2021.166218"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780373"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(22)00260-9"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2022-0520-SA"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22147464"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.10.002"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2016.03.008"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9061194"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1159\/000447845"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.7.7540549"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.5115\/acb.20.234"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/apm.12847"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00300"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.5114\/ceji.2018.81352"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1307681110"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scib.2023.08.031"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.064964"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jix260"},{"issue":"8","key":"e_1_2_9_37_1","first-page":"1345","article-title":"Monkeypox virus in pregnancy, the placenta and newborn: an emerging poxvirus with similarities to smallpox and other orthopoxvirus agents causing maternal and fetal disease","volume":"11","author":"Schwartz DA","year":"2023","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-092917-043236"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines11081345"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.29620","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T19:16:29Z","timestamp":1744917389000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.29620"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4]]},"references-count":38,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.1002\/jmv.29620"],"URL":"https:\/\/doi.org\/10.1002\/jmv.29620","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4]]},"assertion":[{"value":"2023-12-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e29620"}}