{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T03:58:45Z","timestamp":1780113525034,"version":"3.54.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Individuals infected with dengue virus (DENV) often show no symptoms, which raises the risk of DENV transfusion transmission (TT\u2010DENV) in areas where the virus is prevalent. This study aimed to determine the evidence of DENV infection in blood donors from different geographic regions of Thailand. A cross\u2010sectional study was conducted on blood donor samples collected from the Thai Red Cross National Blood Center and four regional blood centers between March and September 2020. Screening for DENV nonstructural protein 1 (NS1), anti\u2010DENV immunoglobulin G (IgG), and IgM antibodies was performed on residual blood from 1053 donors using enzyme\u2010linked immunosorbent assay kits. Positive NS1 and IgM samples indicating acute infection were verified using four different techniques, including quantitative real\u2010time (q) RT\u2010PCR, nested PCR, virus isolation in C6\/36 cells, and mosquito amplification. DENV IgG seropositivity was identified in 89% (938\/1053) of blood donors. Additionally, 0.4% (4\/1053) and 2.1% (22\/1053) of Thai blood donors tested positive for NS1 and IgM, respectively. The presence of asymptomatic dengue virus infection in healthy blood donors suggests a potential risk of transmission through blood transfusion, posing a concern for blood safety.<\/jats:p>","DOI":"10.1002\/jmv.29689","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:50:53Z","timestamp":1717134653000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Dengue virus transmission risk in blood donation: Evidence from Thailand"],"prefix":"10.1002","volume":"96","author":[{"given":"Umaporn","family":"Limothai","sequence":"first","affiliation":[{"name":"Excellence Center for Critical Care Nephrology King Chulalongkorn Memorial Hospital Bangkok Thailand"},{"name":"Center of Excellence in Critical Care Nephrology, Faculty of Medicine Chulalongkorn University Bangkok Thailand"},{"name":"Tropical Medicine Cluster Chulalongkorn University Bangkok 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