{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T21:05:06Z","timestamp":1765487106059,"version":"3.45.0"},"reference-count":62,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The diversity and evolution of the genomes of human bocavirus (HBoV), which causes respiratory diseases, have been scarcely studied. Here, we aimed to obtain and characterize HBoV genomes from patients's nasopharyngeal samples collected between 2017 and 2022 period (5 years and 7 months). Next\u2010generation sequencing (NGS) used Illumina technology after having implemented using GEMI an in\u2010house multiplex PCR amplification strategy. Genomes were assembled and analyzed with CLC Genomics, Mafft, BioEdit, MeV, Nextclade, MEGA, and iTol. A total of 213 genomes were obtained. Phylogeny classified them all as of Bocavirus 1 (HBoV1) species. Five HBoV1 genotypic clusters determined by hierarchical clustering analysis of 27 variable genome positions were scattered over the study period although with differences in yearly prevalence. A total of 167 amino acid substitutions were detected. Besides, coinfection was observed for 52% of the samples, rhinoviruses then adenoviruses (HAdVs) being the most common viruses. Principal component analysis showed that HBoV1 genotypic cluster \u03b1 tended to be correlated with HAdV co\u2010infection. Subsequent HAdV typing for HBoV1\u2010positive samples and negative controls demonstrated that HAdVC species predominated but HAdVB was that significantly HBoV1\u2010associated. Overall, we described here the first HBoV1 genomes sequenced for France. HBoV1 and HAdVB association deserves further investigation.<\/jats:p>","DOI":"10.1002\/jmv.29706","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T06:33:59Z","timestamp":1718692439000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Sequencing and characterization of human bocavirus genomes from patients diagnosed in Southern France between 2017 and 2022"],"prefix":"10.1002","volume":"96","author":[{"given":"Houmadi","family":"Hikmat","sequence":"first","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorlane","family":"Le Targa","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Biosellal Lyon France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celine","family":"Boschi","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justine","family":"Py","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marielle","family":"Bedotto","sequence":"additional","affiliation":[{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lie","family":"Morand","sequence":"additional","affiliation":[{"name":"Service d'Accueil des Urgences P\u00e9diatriques H\u00f4pital Nord, AP\u2010HM Marseille France"},{"name":"Service de P\u00e9diatrie G\u00e9n\u00e9rale H\u00f4pital Timone, AP\u2010HM Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadim","family":"Cassir","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Aherfi","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8006-7704","authenticated-orcid":false,"given":"Bernard","family":"La Scola","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6285-0308","authenticated-orcid":false,"given":"Philippe","family":"Colson","sequence":"additional","affiliation":[{"name":"Microbes Evolution Phylogeny and Infection (MEPHI) Aix\u2010Marseille Universite Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"},{"name":"Assistance Publique\u2010H\u00f4pitaux de Marseille (AP\u2010HM) Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v22.i39.8684"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.10042.1"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx932"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02964-15"},{"key":"e_1_2_10_6_1","unstructured":"ShenWHuman Bocavirus 1 Genome Organization and Replication Mechanism. 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