{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T06:50:18Z","timestamp":1763707818310,"version":"3.45.0"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>\n                      Accumulating evidence has demonstrated that high\u2010risk human papillomaviruses (HR\u2010HPVs) are involved in the etiology of a subset of oropharyngeal squamous cell carcinoma (OPSCC). In this regard, the International Agency for Research on Cancer (IARC) has recommended direct molecular HPV testing. So far, there is no agreement on the most appropriate method for HPV detection on OPSCC formalin\u2010fixed paraffin\u2010embedded (FFPE) materials. In this study, we aimed to evaluate the performance of the high\u2010sensitive SureX HPV assay in OPSCC FFPE tissues compared with LiPA\u201025 and p16\n                      <jats:sup>ink4a<\/jats:sup>\n                      immunostaining. A retrospective series of FFPE primary OPSCC cases were diagnosed between 2008 and 2019 and provided by the Henan Cancer Hospital, China. The level of agreement of two assays was determined using Cohen's Kappa (\u03ba) statistics. A total of 230 FFPE OPSCC samples from tumor resections (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009160) and diagnostic biopsies (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200970) were detected. Sixty\u2010six (28.7%) and 70 (30.4%) samples were identified as HPV\u2010DNA\u2010positive by LiPA\u201025 and SureX, respectively, of which HPV16 was largely the most common type (95.5% vs 94.3%). We found a perfect concordance between LiPA\u201025 and SureX for HPV\u2010DNA status (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.906, 95% CI: 0.875\u20130.937) and for HPV16 (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.925, 95% CI: 0.897\u20130.953). In addition, SureX and p16\n                      <jats:sup>ink4a<\/jats:sup>\n                      immunostaining had a perfect concordance (\n                      <jats:italic>\u03ba<\/jats:italic>\n                      \u2009=\u20090.917, 95% CI: 0.888\u20100.946). Moreover, the HPV\u2010driven fraction, based on double positivity for HPV\u2010DNA and p16\n                      <jats:sup>ink4a<\/jats:sup>\n                      , was similar between SureX (63 of 230, 27.4%) and LiPA\u201025 (60 of 230, 26.1%). Similar results were found in samples from resections and biopsies. SureX and LiPA\u201025 are comparable. SureX could be used for routine HPV\u2010DNA detection and genotyping on archival OPSCC FFPE tissues.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.29716","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T05:51:08Z","timestamp":1717134668000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of an E6\/E7 PCR\u2010capillary electrophoresis fragment analysis in the genotyping of human papillomavirus in archival FFPE samples of oropharyngeal cancer"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4874-3810","authenticated-orcid":false,"given":"Jian","family":"Yin","sequence":"first","affiliation":[{"name":"School of Population Medicine and Public Health Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"},{"name":"Department of Cancer Epidemiology National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yufei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Population Medicine and Public Health Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"},{"name":"Department of Cancer Epidemiology National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4876-8337","authenticated-orcid":false,"given":"Cheng","family":"Song","sequence":"additional","affiliation":[{"name":"School of Population Medicine and Public Health Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cancer Epidemiology The Affiliated Cancer Hospital of Zhengzhou University &amp; Henan Cancer Hospital Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingjing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cancer Epidemiology National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongmei","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology The Affiliated Cancer Hospital of Zhengzhou University &amp; Henan Cancer Hospital Zhengzhou 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