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The present study elucidated the inhibitory effects of human seminal plasma (SP) on Jurkat cell (JC)\u2010mediated mumps virus (MuV) infection. We demonstrated that MuV efficiently infected JCs and that the JCs infected by MuV (JC\u2010MuV) mediated MuV infection of HeLa cells. Remarkably, SP was highly cytotoxic to JCs and inhibited JC\u2010MuV infection of HeLa cells. The cytotoxic factor possessed a molecular weight of less than 3\u2009kDa, whereas that of the viricidal factor was over 100\u2009kDa. The cooperation of cytotoxic and viricidal factors was required for the SP inhibition of JC\u2010MuV infection, and prostatic fluid (PF) was responsible for both the cytotoxic and viricidal effects of SP. The cytotoxic effects we observed were resistant to the treatment of PF with boiling water, proteinase K, RNase A, and DNase I. Our results provide novel insights into the antiviral properties of SP, which may limit cell\u2010mediated sexual viral transmission.<\/jats:p>","DOI":"10.1002\/jmv.29733","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T07:57:29Z","timestamp":1718351849000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cytotoxic and viricidal effects of human semen on mumps virus\u2010infected lymphocytes: In vitro studies"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1116-3142","authenticated-orcid":false,"given":"Fei","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Cell Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College Beijing 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