{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:09:01Z","timestamp":1791216541118,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T00:00:00Z","timestamp":1718323200000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Ebolavirus disease (EVD) is an often\u2010lethal disease caused by the genus Ebolavirus (EBOV). Although vaccines are being developed and recently used, outbreak control still relies on a combination of various factors, including rapid identification of EVD cases. This allows rapid patient isolation and control measure implementation. Ebolavirus diagnosis is performed in treatment centers or reference laboratories, which usually takes a few hours to days to confirm the outbreak or deliver a clear result. A fast and field\u2010deployable molecular detection method, such as the isothermal amplification recombinase\u2010aided amplification (RAA), could significantly reduce sample\u2010to\u2010result time. In this study, a RT\u2010RAA assay was evaluated for EBOV detection. Various primer and probe combinations were screened; analytical sensitivity and cross\u2010specificity were tested. A total of 40 archived samples from the 2014 to 2016 Ebola outbreak in West Africa were tested with both the reference method real\u2010time RT\u2010PCR and the established RT\u2010RAA assay. The assay could detect down to 22.6 molecular copies per microliter. No other pathogens were detected with the Ebolavirus RT\u2010RAA assay. Testing 40 samples yield clinical sensitivity and specificity of 100% each. This rapid isothermal RT\u2010RAA assay can replace the previous RT\u2010RPA and continue to offer rapid EBOV diagnostics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jmv.29744","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T07:54:38Z","timestamp":1718351678000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid detection of Ebolavirus using isothermal recombinase\u2010aided amplification"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3219-8691","authenticated-orcid":false,"given":"Arianna","family":"Ceruti","sequence":"first","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health Leipzig University Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3207-6344","authenticated-orcid":false,"given":"Martin","family":"Faye","sequence":"additional","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar Dakar Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5461-5623","authenticated-orcid":false,"given":"Moussa M.","family":"Diagne","sequence":"additional","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar Dakar Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rea M.","family":"Kobialka","sequence":"additional","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health Leipzig University Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila","family":"Makiala\u2010Mandanda","sequence":"additional","affiliation":[{"name":"Department of Virology at the Institut National de Recherche Biom\u00e9dicale (INRB) Kinshasa Democratic Republic of the Congo"},{"name":"Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of the Congo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ousmane","family":"Faye","sequence":"additional","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar Dakar Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oumar","family":"Faye","sequence":"additional","affiliation":[{"name":"Virology Department Institut Pasteur de Dakar Dakar Senegal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed A.","family":"El Wahed","sequence":"additional","affiliation":[{"name":"Institute of Animal Hygiene and Veterinary Public Health Leipzig University Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Virology Medizinische Hochschule Brandenburg Theodor Fontane Neuruppin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-0147-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7108a2"},{"key":"e_1_2_9_4_1","unstructured":"WHO.Ebola Situation Report. WHO;2015."},{"key":"e_1_2_9_5_1","unstructured":"WHO.Ebola Virus Disease. 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