{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T09:42:20Z","timestamp":1780479740964,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005329","name":"Natural Science Foundation of Guizhou Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human papillomavirus (HPV) type 81 has recently become one of the most common low\u2010risk HPV types; however, literature focusing on it is limited. This study aimed to analyze the reasons for the increased detection rate of HPV81 and investigate its evolving pathogenicity. We analyzed the detection rates and trends of HPV81 in 229 061 exfoliated cervical cell samples collected from 2014 to 2023; collected samples of HPV81 single infections from two different time periods; and analyzed the allele frequencies, positive selection, viral load, persistent infection capacity, and pathogenicity of\n                    <jats:italic>E6<\/jats:italic>\n                    and\n                    <jats:italic>E7<\/jats:italic>\n                    genotypes. We found that the detection rate of HPV81 ranked first among the low\u2010risk types in exfoliated cervical cells and exhibited a significantly increasing trend (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001). The frequency of the\n                    <jats:italic>E6<\/jats:italic>\n                    prototype allele of HPV81 (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009317) was significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.018) and demonstrated the strongest adaptive capacity. The viral load and persistent infection capacity of the\n                    <jats:italic>E6<\/jats:italic>\n                    prototype were significantly higher than those of the mutants, thus serving as key drivers for increasing the detection rate of HPV81 and enhancing its pathogenicity. The viral load was positively correlated with persistent infection capacity and pathogenicity. Persistent infection was a crucial factor in the pathogenicity of HPV81. Successful adaptive evolution of HPV81 is accompanied by enhanced pathogenicity.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29753","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T04:59:11Z","timestamp":1718773151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Study on the detection rate, genetic polymorphism, viral load, persistent infection capacity, and pathogenicity of human papillomavirus type 81"],"prefix":"10.1002","volume":"96","author":[{"given":"Zuyi","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiongyao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Information Affiliated Hospital of Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Affiliated Hospital of Zunyi Medical University Zunyi China"},{"name":"School of Laboratory Medicine Zunyi Medical University Zunyi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Chongqing Tongnan Women and Children Health Care Hospital Chongqing 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