{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T14:52:58Z","timestamp":1767192778540,"version":"3.41.2"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T00:00:00Z","timestamp":1719792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Donor and recipient human cytomegalovirus (HCMV) seropositive (D+R+) lung transplant recipients (LTRs) often harbor multiple strains of HCMV, likely due to transmitted donor (D) strains and reactivated recipient (R) strains. To date, the extent and timely occurrence of each likely source in shaping the post\u2010transplantation (post\u2010Tx) strain population is unknown. Here, we deciphered the D and R origin of the post\u2010Tx HCMV strain composition in blood, bronchoalveolar lavage (BAL), and CD45+ BAL cell subsets. We investigated either D and\/or R formalin\u2010fixed paraffin\u2010embedded blocks or fresh D lung tissue from four D+R+ LTRs obtained before transplantation. HCMV strains were characterized by short amplicon deep sequencing. In two LTRs, we show that the transplanted lung is reseeded by R strains within the first 6 months after transplantation, likely by infiltrating CD14+ CD163+\/\u2212 alveolar macrophages. In three LTRs, we demonstrate both rapid \n<jats:sc>D<\/jats:sc>\u2010strain dissemination and persistence in the transplanted lung for &gt;1 year post\u2010Tx. Broad inter\u2010host diversity contrasts with intra\u2010host genotype sequence stability upon transmission, during follow\u2010up and across compartments. In D+R+ LTRs, HCMV strains of both, D and R origin can emerge first and dominate long\u2010term in subsequent episodes of infection, indicating replication of both sources despite pre\u2010existing immunity.<\/jats:p>","DOI":"10.1002\/jmv.29770","type":"journal-article","created":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T11:44:26Z","timestamp":1719834266000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bidirectional transfer of human cytomegalovirus strains in donor and recipient seropositive lung transplant patients"],"prefix":"10.1002","volume":"96","author":[{"given":"B\u00fcsra","family":"K\u00fclekci","sequence":"first","affiliation":[{"name":"Center for Virology Medical University of Vienna Vienna Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madlen","family":"Mollik","sequence":"additional","affiliation":[{"name":"Center for Virology Medical University of Vienna Vienna 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