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Despite this, an overlooked chikungunya fever outbreak occurred in Yunnan province, China, in 2019 and false negatives are commonly encountered during alphaviruses screening practice, highlighting the need for improved detection methods. In this study, we developed an improved alphaviruses\u2010specific RT\u2010qPCR capable of detecting chikungunya virus, eastern equine encephalitis virus, western equine encephalitis virus, Venezuelan equine encephalitis virus, Sindbis virus, Mayaro virus, and Ross River virus with high sensitivity and specificity. The assay identified three chikungunya virus\u2010positive cases out of 188 sera retrospectively. Later genetic characterization suggested that imported cases from neighboring countries may be responsible for the neglected chikungunya fever outbreak of 2019 in Yunnan. Our findings underscore the value of improved alphaviruses\u2010specific RT\u2010qPCR in bolstering alphaviruses surveillance and informing preventive strategies.<\/jats:p>","DOI":"10.1002\/jmv.29788","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T06:39:06Z","timestamp":1720593546000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["An improved alphaviruses\u2010specific RT\u2010qPCR facilitates monitoring and prevention of alphaviruses"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9074-4238","authenticated-orcid":false,"given":"Lyu","family":"Xie","sequence":"first","affiliation":[{"name":"Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College  Beijing 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