{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T13:56:16Z","timestamp":1787061376017,"version":"build-2736575974"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,9]],"date-time":"2024-07-09T00:00:00Z","timestamp":1720483200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Coxsackievirus A16 (CV\u2010A16) is a significant etiologic agent of hand, foot, and mouth disease (HFMD) and herpangina (HA), with the capacity to progress to severe complications, including encephalitis, aseptic meningitis, acute flaccid paralysis, myocarditis, and other critical conditions. Beijing's epidemiological surveillance system, established in 2008, encompasses 29 hospitals and 16 district disease control centers. From 2019 to 2021, the circulation of CV\u2010A16 was characterized by the co\u2010circulation of B1a and B1b clades. Multiple cases of HFMD linked to clade B1c has not been reported in Beijing until 2022. This study enrolled 400 HFMD and 493 HA cases. Employing real\u2010time RT\u2010PCR, 368 enterovirus\u2010positive cases were identified, with 180 selected for sequencing. CV\u2010A16 was detected in 18.89% (34\/180) of the cases, second only to CV\u2010A6, identified in 63.33% (114\/180). Full\u2010length VP1 gene sequences were successfully amplified and sequenced in 22 cases, revealing the presence of clades B1a, B1b, and B1c in 14, 3, and 5 cases, respectively. A cluster of five B1c clade cases occurred between June 29 and July 17, 2022, within a 7\u2010km diameter region in Shunyi District. Phylogenetic analysis of five complete VP1 gene sequences and two full\u2010genome sequences revealed close clustering with the 2018 Indian strain (GenBank accession: MH780757.1) within the B1c India branch, with NCBI BLAST results showing over 98% similarity. Comparative sequence analysis identified three unique amino acid variations (P3S, V25A, and I235V). The 2022 Shunyi District HFMD cases represent the first instances of spatiotemporally correlated CV\u2010A16 B1c clade infections in Beijing, underscoring the necessity for heightened surveillance of B1c clade CV\u2010A16 in HFMD and HA in this region.<\/jats:p>","DOI":"10.1002\/jmv.29796","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T02:34:56Z","timestamp":1720578896000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["First detection of multiple cases related to CV\u2010A16 strain of B1c clade in Beijing in 2022"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7657-9255","authenticated-orcid":false,"given":"Zhichao","family":"Liang","sequence":"first","affiliation":[{"name":"Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control  Beijing 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Center for Disease Prevention and Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Cui","sequence":"additional","affiliation":[{"name":"Institute for HIV\/AIDS and STD Prevention and Control, Beijing Center for Disease Prevention and Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyong","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute for Infectious Disease and Endemic Disease Control, Beijing Center for Disease Prevention and Control  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"Institute for Infectious 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