{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T09:38:44Z","timestamp":1786268324828,"version":"build-2736575974"},"reference-count":12,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T00:00:00Z","timestamp":1722297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Primary effusion lymphoma (PEL) is a rare B\u2010cell non\u2010Hodgkin lymphoma associated with Kaposi Sarcoma\u2010associated herpesvirus (KSHV\/HHV8) infection. Lymphoma cells are coinfected with Epstein\u2010Barr virus (EBV) in 60\u221280% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow\u2010Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations.<\/jats:p>","DOI":"10.1002\/jmv.29836","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T12:08:23Z","timestamp":1722341303000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Combined Flow\u2010Fluorescence in situ hybridization to HHV\u20108 and EBV reveals the viral heterogeneity of primary effusion lymphoma"],"prefix":"10.1002","volume":"96","author":[{"given":"Romain","family":"Stammler","sequence":"first","affiliation":[{"name":"Inserm U976 HIPI, Institut Universitaire d'H\u00e9matologie Universit\u00e9 Paris Cit\u00e9 Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauriane","family":"Vacher","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, H\u00f4pital Saint Louis, AP\u2010HP Universit\u00e9 Paris Cit\u00e9 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