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EH is complex and multifaceted, involving immunological changes, environmental influences, and genetic aberrations. Certain genetic variants of the thymic stromal lymphopoietin (\n                    <jats:italic>TSLP<\/jats:italic>\n                    ) may predispose to develop severe HSV\u20101\u2010induced eczema. Therefore, we investigated the impact of TSLP on HSV\u20101 infection.\n                    <jats:italic>TSLP<\/jats:italic>\n                    encodes for two distinct forms: a long\u2010form (lfTSLP), primarily associated with type 2 immunity, and a short\u2010form (sfTSLP) with anti\u2010inflammatory and antimicrobial properties. While sfTSLP reduced HSV\u20101 infectibility in human primary keratinocytes (HPK), lfTSLP did not. In HPK treated with sfTSLP, HSV\u20101 gene expression, and replication decreased, while virion binding to cells and targeting of incoming capsids to the nucleus were not diminished compared to untreated cells. sfTSLP caused only minor changes in the expression of innate immunity cytokines, and its inhibition of HSV\u20101 infection did not require\n                    <jats:italic>de novo<\/jats:italic>\n                    protein synthesis. Time window experiments indicated a different antiviral mechanism than LL\u201037. sfTSLP showed the strongest antiviral effect when administered to HPK before or after inoculation with HSV\u20101, and outperformed the inhibitory potential of LL\u201037 under these conditions. Our data show that sfTSLP has antiviral functions and promotes repression of the HSV\u20101 infection in HPK.\n                  <\/jats:p>","DOI":"10.1002\/jmv.29865","type":"journal-article","created":{"date-parts":[[2024,9,5]],"date-time":"2024-09-05T02:52:01Z","timestamp":1725504721000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Short\u2010form thymic stromal lymphopoietin (sfTSLP) restricts herpes simplex virus infection of human primary keratinocytes"],"prefix":"10.1002","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5984-1873","authenticated-orcid":false,"given":"Jana","family":"Zeitvogel","sequence":"first","affiliation":[{"name":"Division of Immunodermatology and Allergy Research, Department of Dermatology and Allergy Hannover Medical School Hannover Germany"},{"name":"Cluster of Excellence RESIST (EXC 2155) Hannover Medical School Hannover 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