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Both N121T and N121S substitutions have been observed in natural conditions and in a hamster model of dual infection with SARS\u2010CoV\u20102 and Influenza A virus. Serum pseudotype neutralization assays against HIV\u20101 particles carrying wild\u2010type, N121T, and N121S spikes with immune mouse and human sera revealed that N121T and N121S mutations had a greater impact on serum neutralization than biliverdin treatment. Although N121T and N121S substitutions are not currently major SARS\u2010CoV\u20102 variants of concern, this study could provide fundamental information to prepare for potential future mutations at the N121 site of SARS\u2010CoV\u20102.<\/jats:p>","DOI":"10.1002\/jmv.29871","type":"journal-article","created":{"date-parts":[[2024,9,2]],"date-time":"2024-09-02T05:08:36Z","timestamp":1725253716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["N121T and N121S substitutions on the SARS\u2010CoV\u20102 spike protein impact on serum neutralization"],"prefix":"10.1002","volume":"96","author":[{"given":"Van Thi","family":"Lo","sequence":"first","affiliation":[{"name":"Department of Biological Science and Biotechnology College of Natural Science, Chungbuk National University Cheongju South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun 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