{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:18Z","timestamp":1786978098150,"version":"build-2736575974"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T00:00:00Z","timestamp":1731369600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Enterovirus A71<\/jats:italic>\n                    (EVA71) is a leading causative agent of hand, foot, and mouth disease, posing a significant threat to the health of young children, particularly in the Asia\u2010Pacific region. Currently, there is no specific antiviral drug for EVA71 infection; therefore, early and rapid diagnosis is critical for disease prevention and control. Here, we report the development of a simple, rapid, and sensitive detection method for EVA71 infection using reverse transcription\u2010multiple cross displacement amplification (RT\u2010MCDA) combined with nanoparticle\u2010based lateral flow biosensors (LFB). In the RT\u2010MCDA system, a set of 10 primers was designed to target the highly conserved region of the VP1 gene of EVA71 and amplify the genes in an isothermal amplification device. The RT\u2010MCDA amplification reaction products could then be identified by visual detection reagent (VDR) and LFB without the need for specialized equipment. The results demonstrated that the optimal reaction condition for the EVA71\u2010RT\u2010MCDA assay was 65\u2103 for 40 min. The EVA71\u2010RT\u2010MCDA assay could detect as low as 40 copies of plasmid and 50 copies of pseudotyped virus in a reaction. No cross\u2010reaction was found between EVA71 strains and non\u2010EVA71 strains. For 125 clinical anal swab samples, with EVA71\u2010RT\u2010MCDA assay, 30 samples were positive, which was in consistent with the the conventional real\u2010time quantitative reverse transcription polymerase chain reaction assays. The entire procedure, including a 15\u2010min specimen processing step, a 40\u2010min MCDA reaction, and result reporting within 2 min, was completed in less than 60 min. In conclusion, the EVA71\u2010RT\u2010MCDA\u2010LFB assay targeting the VP1 gene is a rapid, highly sensitive, simple, and specific test that could be widely applied in point\u2010of\u2010care settings and basic medical facilities in rural areas.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70059","type":"journal-article","created":{"date-parts":[[2024,11,12]],"date-time":"2024-11-12T11:23:57Z","timestamp":1731410637000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Highly Sensitive and Specific Diagnosis of\n                    <i>Enterovirus A71<\/i>\n                    by Reverse Transcription Multiple Cross\u2010Displacement Amplification\u2010Labeled Nanoparticles Biosensor"],"prefix":"10.1002","volume":"96","author":[{"given":"Jinzhi","family":"Cheng","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences Guizhou Medical University Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical laboratory The First People's Hospital of Guiyang Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomin","family":"Tang","sequence":"additional","affiliation":[{"name":"Laboratory of Bacterial Infectious Disease of Experimental Center Guizhou Provincial Centre for Disease Control and Prevention Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingrun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Clinical laboratory The First People's Hospital of Guiyang Guiyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6756-8301","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical laboratory The First People's 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