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Therefore, it is essential to investigate the impact of COVID\u201019 on pre\u2010existing conditions in patients, such as cancer and other infectious diseases. Recent clinical studies have reported the reactivation of human herpesviruses, including Kaposi's sarcoma\u2010associated herpesvirus (KSHV), in severe COVID\u201019 patients or vaccinated individuals. To support these clinical observations, we established a KSHV\/SARS\u2010CoV\u20102 co\u2010infection system in A549\u2010hACE2 cells. Our findings indicate that co\u2010infection with live SARS\u2010CoV\u20102 sharply induces KSHV lytic reactivation. Transcriptomic analysis revealed significant changes in global cellular gene expression in KSHV\u2010infected A549\u2010hACE2 cells, both with and without SARS\u2010CoV\u20102 co\u2010infection. These data provide a molecular basis for understanding whether patients with pre\u2010existing oncogenic herpesvirus infections are at increased risk for more severe COVID\u201019 or for developing virus\u2010associated cancers even after full recovery from COVID\u201019.<\/jats:p>","DOI":"10.1002\/jmv.70149","type":"journal-article","created":{"date-parts":[[2024,12,30]],"date-time":"2024-12-30T12:04:18Z","timestamp":1735560258000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Alterations in Cellular Gene Expression Due to Co\u2010Infection With Kaposi's Sarcoma\u2010Associated Herpesvirus and SARS\u2010CoV\u20102: Implications for Disease Severity"],"prefix":"10.1002","volume":"97","author":[{"given":"Jungang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathology, Winthrop P. 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