{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T18:55:49Z","timestamp":1773860149125,"version":"3.50.1"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,18]],"date-time":"2025-01-18T00:00:00Z","timestamp":1737158400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>An unprecedented global outbreak caused by the monkeypox virus (MPXV) prompted the World Health Organization to declare a public health emergency of international concern on July 23, 2022. Therapeutics and vaccines for MPXV are not widely available, necessitating further studies, particularly in drug repurposing area. To this end, the standardization of in vitro infection systems is essential. The most robust in vitro studies on poxviruses concern the Vaccinia virus, and there are significant gaps in understanding the replicative cycle of MPXV. Herein, we conducted ultrastructural studies using transmission and scanning electron microscopies and 3D reconstruction to describe and elucidate the step\u2010by\u2010step morphogenesis of MPXV. Vero cells, derived from the kidney lineage of Cercopithecus aethiops monkeys, were infected with a strain isolated from an oropharyngeal swab of a patient with suspected Mpox, collected during an observational cohort study conducted between June 12 and August 19, 2022, in Rio de Janeiro, Brazil. Infected Vero cells exhibited several morphological alterations, including cell lysis plaque formation, nuclei with altered chromatin profiles, thickening of the rough endoplasmic reticulum (RER), presence of myelin figures, disorganization of mitochondrial cristae, and the formation of a granular and fibrous matrix (viral factory) surrounded by mitochondria and RER cisternae in a perinuclear space. Viral entry into cells occurred via endocytosis MPXV particles were observed adhering to cytoskeletal filaments, and viral progeny extrusion occurred through exocytosis. This article presents novel data on the morphogenesis of MPXV that have not been previously documented in the literature.<\/jats:p>","DOI":"10.1002\/jmv.70180","type":"journal-article","created":{"date-parts":[[2025,1,18]],"date-time":"2025-01-18T11:42:22Z","timestamp":1737200542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["MPXV: Update on Morphological and Morphogenesis Aspects Through Transmission and Scanning Electron Microscopies and 3D Reconstruction"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1014-0755","authenticated-orcid":false,"given":"Debora Ferreira","family":"Barreto\u2010Vieira","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0037-059X","authenticated-orcid":false,"given":"Milene Dias","family":"Miranda","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1707-6525","authenticated-orcid":false,"given":"Marcos Alexandre Nunes","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6838-0167","authenticated-orcid":false,"given":"Andressa Santos","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2189-7115","authenticated-orcid":false,"given":"Ana Luisa Teixeira","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6591-4987","authenticated-orcid":false,"given":"Derick Mendes","family":"Bandeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1712-2672","authenticated-orcid":false,"given":"Vivian Neuza S.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1350-1301","authenticated-orcid":false,"given":"Alice Santos","family":"Rosa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8186-8714","authenticated-orcid":false,"given":"Wendell","family":"Girard\u2010Dias","sequence":"additional","affiliation":[{"name":"Plataforma de Microscopia Eletr\u00f4nica Rudolf Barth Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8145-7712","authenticated-orcid":false,"given":"Br\u00e1ulio Soares","family":"Archanjo","sequence":"additional","affiliation":[{"name":"N\u00facleo de Laborat\u00f3rios de Microscopia Instituto Nacional de Metrologia, Qualidade e Tecnologia Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2418-8605","authenticated-orcid":false,"given":"Ortrud Monika","family":"Barth","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Morfologia e Morfog\u00eanese Viral Instituto Oswaldo Cruz, Funda\u00e7\u00e3o Oswaldo Cruz\u2010Fiocruz Rio de Janeiro Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit703"},{"key":"e_1_2_10_3_1","unstructured":"International Commitee on Taxonomy of Viruses\u2013Poxviridae. 2024 https:\/\/ictv.global\/report\/chapter\/poxviridae\/poxviridae\/orthopoxvirus."},{"issue":"5","key":"e_1_2_10_4_1","first-page":"593","article-title":"A Human Infection Caused by Monkeypox Virus in Basankusu Territory, Democratic Republic of the Congo","volume":"46","author":"Ladnyj I. D.","year":"1972","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_10_5_1","unstructured":"Centers for Disease Control and Prevention (CDC) Monkeypox Outbreak Global Map. 2024 https:\/\/www.cdc.gov\/poxvirus\/monkeypox\/response\/2022\/world-map.html."},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2021.26.32.2100745"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.add4153"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)01437-4"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2022.2121420"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed7100264"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14092012"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28493"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2444"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00639-z"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajem.2023.01.007"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jtm\/taac077"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.106759"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/BC20070173"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201600476"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2019"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3791\/57199"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/v10040142"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00027-09"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/v7122940"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42649-022-00080-3"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11071781"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lana.2022.100406"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-02760230090"},{"key":"e_1_2_10_29_1","volume-title":"Biosafety in Microbiological and Biomedical Laboratories","author":"CDC \u2010 Centers for Disease Control and Prevention","year":"2020"},{"key":"e_1_2_10_30_1","unstructured":"D. F.Barreto\u2010Vieira O. M.Barth\u2010Schatzmayr andH. G.SchatzmayrManual de T\u00e9cnicas. Interci\u00eancia; Rio de Janeiro Brasil: 2010. Modelo animal experimental para o estudo da patog\u00eanese dos v\u00edrus dengue sorotipos 1 e 2."},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1590\/0074-027601504"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0013"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14020201"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0703.010311"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.20-0589"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-022-01874-z"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01336-22"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.24171\/j.phrp.2022.0232"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.46234\/ccdcw2022.206"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2023.2208682"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2023.1195674"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28911"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2023.03.009"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.28536"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00381.x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0303-6987.2005.00254.x"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.53854\/liim-3201-10"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80831-0"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01053-06"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004618107"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90333-5"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.02.018"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000785"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/378636a0"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.109.7.1739"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003239"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1183173"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.11.004"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T08:26:23Z","timestamp":1741767983000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":57,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1002\/jmv.70180"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70180","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70180"}}