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The aim of this study was to investigate whether the choroid plexus could serve as a potential entry site for SARS\u2010CoV\u20102 into the brain. Tissue samples from 24 deceased COVID\u201019\u2010positive individuals were analyzed. Reverse transcription real\u2010time PCR (RT\u2010qPCR) was performed on selected brain regions, including the choroid plexus, to detect SARS\u2010CoV\u20102 viral RNA. Additionally, immunofluorescence staining and confocal microscopy were used to detect and localize two characteristic proteins of SARS\u2010CoV\u20102: the spike protein S1 and the nucleocapsid protein. RT\u2010qPCR analysis confirmed the presence of SARS\u2010CoV\u20102 viral RNA in the choroid plexus. Immunohistochemical staining revealed viral particles localized in the epithelial cells of the choroid plexus, with the spike protein S1 detected in the late endosomes. Our findings suggest that the blood\u2010cerebrospinal fluid (B\u2010CSF) barrier in the choroid plexus serves as a route of entry for SARS\u2010CoV\u20102 into the CNS. This study contributes to the understanding of the mechanisms underlying CNS involvement in COVID\u201019 and highlights the importance of further research to explore potential therapeutic strategies targeting this entry pathway.<\/jats:p>","DOI":"10.1002\/jmv.70184","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T12:15:16Z","timestamp":1737461716000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The Blood\u2010Cerebrospinal Fluid Barrier as a Potential Entry Site for the SARS\u2010CoV\u20102 Virus"],"prefix":"10.1002","volume":"97","author":[{"given":"Peter","family":"Sol\u00e1r","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine Masaryk University Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"\u0160er\u00fd","sequence":"additional","affiliation":[{"name":"Department of Forensic 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