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The present study aimed to demonstrate that the presence of atypical cells with large vacuoles in the cytoplasm of parabasal cells, referred to as vacuolated parabasal cells (VPCs), which are observed in the Pap smears of HPV\u2010positive women, is associated with specific HPV genotypes. Among 2175 patients, 310 with a single HR\u2010HPV infection and cytological diagnosis of high\u2010grade squamous intraepithelial lesions (HSIL) or atypical squamous cells not excluding HSIL (ASC\u2010H) were included, of which 86 were infected with HPV16. Biopsy results revealed that 69 (80.2%) patients had cervical intraepithelial neoplasia Grade 2 or higher (CIN2+). VPCs were found in 47 (54.7%) of HPV16\u2010infected cases, indicating a significant increase of VPCs in HPV16\u2010infected cases (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01). Episomal HPV16 load was quantified in 142 VPCs and 156 HSIL (ASC\u2010H) cells using liquid\u2010based cytology samples from five patients, with a median of 987 copies in VPCs, significantly higher than those in HSIL (ASC\u2010H) cells (176 copies; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). VPCs in Pap specimens were identified not only as cells altered by HPV16 infection but also as CIN2+\u2010derived cells and HPV16\u2010producing cells.<\/jats:p>","DOI":"10.1002\/jmv.70191","type":"journal-article","created":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T11:59:46Z","timestamp":1738065586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Vacuolated Parabasal Cells in Papanicolaou Smears Are Cellular Changes Caused by Human Papillomavirus 16 Infection"],"prefix":"10.1002","volume":"97","author":[{"given":"Shuichi","family":"Mizuno","sequence":"first","affiliation":[{"name":"Department of Medical Technology Faculty of Health Sciences, Kyorin University Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5994-9670","authenticated-orcid":false,"given":"Kaori","family":"Okayama","sequence":"additional","affiliation":[{"name":"Department of 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