{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:43:37Z","timestamp":1790934217252,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T00:00:00Z","timestamp":1739232000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Enteric viruses are significantly associated with acute gastroenteritis globally. Despite a decrease in severe rotavirus associated diarrhoea, Ghana still records high diarrhoea burden. Meanwhile aetiological investigations in hospital settings do not routinely include viral testing. Rotavirus vaccination is thought to alter enteric viral populations and impact evolution. To better understand virus\u2010specific effects in acute gastroenteritis in both children and adults, we tested fecal samples from 228 patients at two hospitals in Accra from January to December 2019, using multiplex and singleplex PCR assays. The clinical impact of detected viruses was assessed using a modified Vesikari score system. Partial viral genome sequences were obtained by Sanger Sequencing and their genetic diversity and evolutionary history, traced by phylogenetic analyses. At least one enteric virus was found in 86 (37.7%) patient samples, with 36.9% of the population under five infected. Single infections of rotavirus, norovirus, adenovirus, sapovirus and astrovirus were 33, 14, 8, 6, and 1, respectively, while coinfections were 24. Rotavirus accounted for 33.3% of 24 clinically severe cases (modified Vesikari score &gt;\u20097). Three out of 10 rotavirus cases with evidence of vaccination experienced severe gastroenteritis. Diverse genotypes, including RVA G2P[4], G1P[8], G12P[8] and G12P[6]; AdV F40 and F41; NoV GII.4 Sydney 2012, GII.6 and GI.3, several of which clustered with contemporary strains from the Americas, Europe and Asia, were detected. This study also provides the first report of SaV GI.1, GI.7 and GII.8 detection in humans in Ghana. RVA G2P[4] and AdV F were associated with higher proportions of hospitalizations. While RVA continues to have a profound clinical impact on gastroenteritis, AdV and SaV produce an equally severe disease. In contrast, NoV and AstV showed a generally mild to moderate impact on clinical disease severity.<\/jats:p>","DOI":"10.1002\/jmv.70216","type":"journal-article","created":{"date-parts":[[2025,2,12]],"date-time":"2025-02-12T06:37:03Z","timestamp":1739342223000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical Impact and Genetic Analysis of Enteric Viruses Associated With Acute Gastroenteritis in Greater Accra, Ghana: A Comprehensive Study of Five Viruses"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-1729","authenticated-orcid":false,"given":"Lawrence Henry","family":"Ofosu\u2010Appiah","sequence":"first","affiliation":[{"name":"Department of Environmental Medicine Kochi Medical School, Kochi University Kochi Japan"},{"name":"National Public Health and Reference Laboratory, Public Health Division Ghana Health Service Accra Ghana"},{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manami","family":"Negoro","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer Xolali","family":"Amexo","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dodzi Kofi","family":"Amelor","sequence":"additional","affiliation":[{"name":"National Public Health and Reference Laboratory, Public Health Division Ghana Health Service Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prince Baffour","family":"Tonto","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis Odai","family":"Laryea","sequence":"additional","affiliation":[{"name":"Disease Surveillance Department, Public Health Division Ghana Health Service Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiko","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine Kochi Medical School, Kochi University Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin","family":"Asiedu\u2010Bekoe","sequence":"additional","affiliation":[{"name":"Disease Surveillance Department, Public Health Division Ghana Health Service Accra Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Sugata","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Medical Education Graduate School of Medicine Mie University Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narufumi","family":"Suganuma","sequence":"additional","affiliation":[{"name":"Department of Environmental Medicine Kochi Medical School, Kochi University Kochi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyosu","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Department of Pediatric Infectious Diseases Institute for Clinical Research, Mie National Hospital Organization Tsu Mie Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmjgh-2022-009548"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31128-0"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2018.1960"},{"key":"e_1_2_11_5_1","doi-asserted-by":"crossref","unstructured":"C.Arena J. 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