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In this study, 93 CD4<jats:sup>+<\/jats:sup> T\u2010cell epitopes restricted by 12 prevalent HLA\u2010DRB1 allotypes, which covering over 80% Chinese and Northeast Asian populations, were identified from the S, E, M, N and RdRp proteins of SARS\u2010CoV\u20102 by in silico prediction, DC\u2010peptide\u2010PBL coculture experiment, and immunization in HLA\u2010A2\/DR1 transgenic mice. Furthermore, by using validated 215 CD8<jats:sup>+<\/jats:sup> T cell epitope peptides and 123 CD4<jats:sup>+<\/jats:sup> T\u2010cell epitope peptides covering Northeast Asian cohort, the universal ELISpot detection systems of SARS\u2010CoV\u20102 specific CD8<jats:sup>+<\/jats:sup> T cells and CD4<jats:sup>+<\/jats:sup> T cells were established, for the first time, and followed by the tests for 50 unexposed and 100 convalescent samples. The median of spot\u2010forming units for CD8<jats:sup>+<\/jats:sup> T cells and CD4<jats:sup>+<\/jats:sup> T cells were 68 and 15, respectively, in the unexposed donors, but were 137 and 52 in the convalescent donors 6 months after recovery while 128 and 47 in the convalescent donors 18 months after recovery. This work initially provided the broad\u2010spectrum CD4<jats:sup>+<\/jats:sup> T\u2010cell epitope library of SARS\u2010CoV\u20102 for the design of T cell\u2010directed vaccines and the universal T cell detection tool tailoring to Northeast Asian population, and confirmed the long\u2010term memory T cell immunity after SARS\u2010CoV\u20102 infection.<\/jats:p>","DOI":"10.1002\/jmv.70241","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T18:10:31Z","timestamp":1740075031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Large\u2010Scale Screening of CD4<sup>+<\/sup> T\u2010Cell Epitopes From SARS\u2010CoV\u20102 Proteins and the Universal Detection of SARS\u2010CoV\u20102 Specific T Cells for Northeast Asian Population"],"prefix":"10.1002","volume":"97","author":[{"given":"Yu","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology 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