{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:51:48Z","timestamp":1790045508431,"version":"4.0.1"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T00:00:00Z","timestamp":1740009600000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Since the global outbreak of Mpox in 2022, interest in seroepidemiological and vaccine studies has grown rapidly. One aspect of interest in these studies is the detection of neutralizing antibodies to the causative agent, monkeypox virus (MPXV). Determination of neutralizing antibodies using a (micro)\u2010neutralization test (NT) is the gold standard method, which is time consuming and labor intensive. Here, we present the comparison of different methods to facilitate MPXV NT analysis resulting in the establishment of an optimized MPXV NT protocol. The optimized protocol is based on a recombinant eGFP\u2010expressing MPXV and a recombinant mCherry\u2010expressing VeroE6 cell line. Using the so\u2010called Xmas\u2010NT, the incubation time can be reduced by 60%. In addition, compared to the conventional NT, the documentation time of the Xmas\u2010NT is significantly reduced by 80%, making it suitable for serological high throughput screening studies.<\/jats:p>","DOI":"10.1002\/jmv.70246","type":"journal-article","created":{"date-parts":[[2025,2,20]],"date-time":"2025-02-20T18:24:11Z","timestamp":1740075851000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Xmas\u2010NT: A Novel Two\u2010Color Fluorescence Micro Neutralization Test for the Detection of Neutralizing Antibodies Against Monkeypox Virus"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2876-8682","authenticated-orcid":false,"given":"Clemens","family":"Bodenstein","sequence":"first","affiliation":[{"name":"Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens WHO Collaboration Center for Emerging Threats and Special Pathogens, Robert Koch Institute Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5075-8870","authenticated-orcid":false,"given":"Daniel","family":"Bourquain","sequence":"additional","affiliation":[{"name":"Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens WHO Collaboration Center for Emerging Threats and Special Pathogens, Robert Koch Institute Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8185-3176","authenticated-orcid":false,"given":"Andreas","family":"Nitsche","sequence":"additional","affiliation":[{"name":"Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens WHO Collaboration Center for Emerging Threats and Special Pathogens, Robert Koch Institute Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9369-8203","authenticated-orcid":false,"given":"Marica","family":"Grossegesse","sequence":"additional","affiliation":[{"name":"Highly Pathogenic Viruses (ZBS 1), Centre for Biological Threats and Special Pathogens WHO Collaboration Center for Emerging Threats and Special Pathogens, Robert Koch Institute Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,20]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"13","article-title":"Monkeypox: A Comprehensive Review on Mutation, Transmission, Pathophysiology, and Therapeutics","volume":"146","author":"Dutta S.","year":"2024","journal-title":"International Immunopharmacology"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-022-00775-4"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-024-03130-3"},{"key":"e_1_2_9_5_1","unstructured":"WHO. 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