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In addition, HERV transcriptional modulation is of great interest to clarify the etiology of complex disorders such as cancer, even if a few studies assessed the specific HERV loci modulated in tumor tissues. In the present work, we used a transcriptomic approach to investigate the specific expression of ~3300 HERV loci in paired tumor and normal tissues of 7 colorectal cancer (CRC) patients. A total of 102 HERVs were significantly modulated in CRC, with a general tendency towards downregulation. Of note, among the 42 upregulated HERVs 23 belonged to the HERV\u2010H group, that is the most investigated in CRC. De novo transcriptome reconstruction and qPCR validation allowed to identify a transcript from a HERV\u2010H locus on chromosome Xp22.3 with high specific expression in CRC samples, potentially encoding for a partial Pol protein. These results provide a detailed description of HERV transcriptional variations in CRC and its interindividual variability, identifying a HERV\u2010H transcript that deserves further investigation for its possible impact on tumor progression.<\/jats:p>","DOI":"10.1002\/jmv.70249","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T20:03:23Z","timestamp":1740513803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["HERV Modulation in Colorectal Carcinoma Patients: A Snapshot of Endogenous Retroviral Transcriptome"],"prefix":"10.1002","volume":"97","author":[{"given":"Nicole","family":"Grandi","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Virology, Department of Life and Environmental Sciences University of Cagliari Cagliari Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Hsuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, College of Medicine Taipei Medical University Taipei Taiwan"},{"name":"Department of Microbiology &amp; 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