{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:14:32Z","timestamp":1788484472530,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Multidrug\u2010resistant HIV patients have limited ART options. Lenacapavir (LEN) is a capsid inhibitor that exhibits substantial antiviral activity in patients with therapeutic failure but is also proposed for PrEP. Herein, we assessed LEN susceptibility among ART\u2010naive HIV patients with drug resistance in Mozambique. In this study, 63 patients with DRM against PIs, NRTIs, NNRTIs, and INSTIs were included. The gag (p24) and env fragments were amplified with a low\u2010cost in\u2010house protocol and sequenced with nanopore. HIVDR database from Stanford University was used to assess LEN resistance and geno2pheno to assess viral tropism and protease\/maturation inhibitor\u2010associated mutations. A total of 59 patients were successfully sequenced. About 29% had DRMs to PIs, 5% to NRTI, 83% to NNRTI, and 2% to INSTI. No DRMs to LEN were detected. Additionally, 42% of the sequences presented protease\/maturation inhibitor\u2010associated mutations. A relationship was observed between the E138A\/G mutation and protease\/maturation inhibitors (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.004). We identified changes at the first codon position of position 56 of the p24\n                    <jats:italic>gag<\/jats:italic>\n                    gene, which represents a key site for resistance to LEN. Also, codon 66 was highly conserved. Our results support the potential effectiveness of lenacapavir as a PrEP regimen or rescue therapy for patients with at least one drug\u2010resistance mutation.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70317","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T05:36:10Z","timestamp":1742448970000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Susceptibility to Lenacapavir Among Newly Diagnosed HIV\u2010Positive Patients Followed Up in Mozambique That Presented With Primary Antiretroviral Resistance to Other Classes"],"prefix":"10.1002","volume":"97","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1232-0119","authenticated-orcid":false,"given":"Cruz S.","family":"Sebasti\u00e3o","sequence":"first","affiliation":[{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"},{"name":"Centro Nacional de Investiga\u00e7\u00e3o Cient\u00edfica (CNIC) Luanda Angola"},{"name":"Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Angola (CISA) Caxito Angola"},{"name":"Instituto Nacional de Investiga\u00e7\u00e3o em Sa\u00fade (INIS) Luanda Angola"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamila","family":"Bathy","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Manhi\u00e7a (CISM) Manhi\u00e7a Mozambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tacilta","family":"Nhampossa","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Manhi\u00e7a (CISM) Manhi\u00e7a Mozambique"},{"name":"Instituto Nacional de Sa\u00fade, Minist\u00e9rio da Sa\u00fade (INS) Maputo Mo\u00e7ambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9","family":"Santos","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mafalda","family":"Miranda","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neusa Magode","family":"Manhi\u00e7a","sequence":"additional","affiliation":[{"name":"Servi\u00e7o Distrital de Sa\u00fade, Mulher e A\u00e7\u00e3o Social (SDSMAS) de Manhi\u00e7a Manhi\u00e7a Mo\u00e7ambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e3o","family":"Bila","sequence":"additional","affiliation":[{"name":"Servi\u00e7o Distrital de Sa\u00fade, Mulher e A\u00e7\u00e3o Social (SDSMAS) de Manhi\u00e7a Manhi\u00e7a Mo\u00e7ambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delfino","family":"Vubil","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o em Sa\u00fade de Manhi\u00e7a (CISM) Manhi\u00e7a Mozambique"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Seabra","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Ros\u00e1rio","family":"O. Martins","sequence":"additional","affiliation":[{"name":"Global Health and Tropical Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5849-7326","authenticated-orcid":false,"given":"Marta","family":"Giovanetti","sequence":"additional","affiliation":[{"name":"Department of Science and Technology for Humans and the Environment University of Campus Bio\u2010Medico di Roma Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perpetua","family":"Gomes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular (LMCBM, SPC, CHLO\u2010HEM) Lisbon Portugal"},{"name":"Egas Moniz Center for Interdisciplinary Research (CiiEM), Egas Moniz School of Health &amp; Science Caparica Almada 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Medicine (GHTM), Associate Laboratory in Translation and Innovation Towards Global Health (LA\u2010REAL), Instituto de Higiene e Medicina Tropical (IHMT) Universidade NOVA de Lisboa (UNL) Lisboa Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,20]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"WHO Clinical Guidelines: Antiretroviral Therapy(WHO2016) 129."},{"key":"e_1_2_9_3_1","unstructured":"WHO Global Health Sector Strategies on Respectively HIV Viral Hepatitis and Sexually Transmitted Infections for the Period 2022\u20102030 accessed March 15 2024 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