{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:48:10Z","timestamp":1786978090114,"version":"build-2736575974"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hepatitis B virus (HBV) infection is a principal cause of severe liver disease in humans and is associated with increased levels of specific serum or intracellular microRNAs (miRNAs). Among these, miR\u2010193b\u20103p is a liver\u2010enriched miRNA; however, its role in HBV replication remains unknown. This study aimed to investigate the influence of chronic HBV infection on miR\u2010193b\u20103p levels in the peripheral blood and liver tissues of patients with chronic hepatitis B (CHB), evaluate the effect of miR\u2010193b\u20103p on HBV replication both in vitro and in vivo, and elucidate the potential underlying mechanisms. We showed that hepatic miR\u2010193b\u20103p levels in patients with CHB were significantly elevated compared with those in healthy controls. Ectopic expression of miR\u2010193b\u20103p significantly enhanced HBV replication and transcription in different hepatoma cell lines. Furthermore, we identified\n                    <jats:italic>IGF\u20101R<\/jats:italic>\n                    as a direct target through which miR\u2010193b\u20103p regulates HBV replication. Mechanistically, miR\u2010193b\u20103p increased HBV core promoter activity via the IGF\u20101R\/FXR\u03b1 axis, thereby enhancing HBV transcription. Additionally, miR\u2010193b\u20103p increased IGF\u20101R\/Akt\/MDM2\/p53 signaling\u2010mediated autophagy induction, which in turn facilitated increased HBV post\u2010transcriptional activity. Collectively, hepatocyte\u2010enriched miR\u2010193b\u20103p exerts a proviral effect on HBV replication through dual synergistic mechanisms, offering novel insights into its role in HBV replication and potential therapeutic implications in CHB infection.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70330","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:11:41Z","timestamp":1744089101000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Hepatocyte\u2010Enriched miRNA\u2010193b\u20103p Promotes Hepatitis B Virus Replication by Dual Activation of Viral Core Promoter Activity and Autophagy Induction by Targeting IGF\u20101R"],"prefix":"10.1002","volume":"97","author":[{"given":"Yingying","family":"Deng","sequence":"first","affiliation":[{"name":"Key Laboratory of Molecular Biology of Infectious Diseases (Chinese Ministry of Education) Chongqing Medical University No. 1, 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