{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T02:54:44Z","timestamp":1785207284745,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T00:00:00Z","timestamp":1744675200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    We compared the performance of the VIDAS Cytomegalovirus (CMV)\u2010Interferon\u2010gamma release assay (IGRA) with that of laboratory\u2010developed flow cytometry for intracellular cytokine staining (FC\u2010ICS) for the assessment of CMV\u2010specific interferon\u2010gamma (IFN\u2010\u03b3)\u2010producing T\u2010cell responses (CMV\u2010CMI). A total of 147 blood specimens from 78 adult participants were collected: 11 healthy controls, 34 hematological patients (HP), of which 32 had undergone allogeneic hematopoietic cell transplantation (allo\u2010HCT), and 33 Kidney transplant recipients. Of the 147 specimens, 96 tested positive, 24 negative, 25 indeterminate, and 2 were invalid by the VIDAS CMV IGRA. A total of 137 specimens were tested by FC\u2010ICS, of which 107 returned positive results. There were 27 discrepancies across the assays among specimens yielding interpretable results, of which 14 tested VIDAS CMV IGRA\u2010positive\/FC\u2010ICS\u2010negative and 12 VIDAS CMV IGRA\u2010negative\/FC\u2010ICS\u2010positive. The overall agreement between immunoassays was 78%, and the Kappa coefficient was 0.34 (0.52 for HP). Differences in identifying CMV\u2010infected (CMV IgG\u2010positive) and uninfected participants (CMV IgG\u2010negative) were noticed across both assays. The overall correlation (rho values) between IFN\u2010\u03b3 concentrations (IU\/ml) measured by the VIDAS CMV IGRA and CMV\u2010specific IFN\u2010\u03b3\u2010producing T\u2010cell frequencies were 0.27 for CD4\n                    <jats:sup>+<\/jats:sup>\n                    and 0.33 for CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.001). In HP, the correlation was stronger (0.48 for CD4\n                    <jats:sup>+<\/jats:sup>\n                    and 0.49 for CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells). In summary, our data lend support to the potential utility of the VIDAS CMV IGRA to assess CMV\u2010CMI in transplant recipients.\n                  <\/jats:p>","DOI":"10.1002\/jmv.70355","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T10:45:41Z","timestamp":1744713941000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of the VIDAS Cytomegalovirus (CMV)\u2010IGRA for Assessing CMV\u2010Specific T\u2010Cell Responses in Immunocompetent and Immunosuppressed Individuals"],"prefix":"10.1002","volume":"97","author":[{"given":"Estela","family":"Gim\u00e9nez","sequence":"first","affiliation":[{"name":"Microbiology Service Clinic University Hospital, INCLIVA Health Research Institute Valencia Spain"},{"name":"CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III Madrid 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