{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:22:21Z","timestamp":1789514541462,"version":"build-2803163510"},"reference-count":43,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,4,22]],"date-time":"2025-04-22T00:00:00Z","timestamp":1745280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Medical Virology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Zika virus (ZIKV) is a neurotropic virus that can be transmitted congenitally. In ZIKV\u2010infected pregnant women, placental dysfunction is associated with the secretion of nonstructural protein 1 (NS1). In this study, the kinetics of NS1 secretion and antibody response were assessed and characterized in the serum of ZIKV\u2010positive adult patients recruited in French Guiana. NS1 concentrations were quantified by a single molecule array (SiMoA) in 164 sequential serum samples collected from thirty patients during the first month after onset of symptoms. Serum NS1 concentrations in this cohort were unexpectedly low and ranged from 0.1\u2009pg\/mL to 380\u2009pg\/mL. The median persistence of NS1 in patients with a clinical score of 2 (6 days) was significantly lower than in patients with a clinical score of 3 (8 days). In both groups of patients, anti\u2010NS1 IgM and IgG kinetics were similar but patients with a milder clinical score of 2 had statistically higher levels of specific IgM than those with a clinical score of 3. Herein, it was shown that NS1 circulating in patient sera is associated with clinical outcome, emphasizing the role of NS1 in ZIKV pathogenesis.<\/jats:p>","DOI":"10.1002\/jmv.70368","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T01:29:42Z","timestamp":1745371782000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Prolonged Zika Virus NS1 Protein Circulation in Patient Sera Impacts Clinical Outcome Before the Rise of a Specific IgM Response"],"prefix":"10.1002","volume":"97","author":[{"given":"Carole","family":"Tamietti","sequence":"first","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Unit\u00e9 de Virologie Structurale Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tharshana","family":"Stephen","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Single Cell Biomarkers UTechS Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Rouvinski","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Unit\u00e9 de Virologie Structurale Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Tenebray","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de virologie Institut de Recherche Biom\u00e9dicale des Arm\u00e9es Marseille France"},{"name":"Centre National de R\u00e9f\u00e9rence des Arbovirus Institut de Recherche Biom\u00e9dicale des Arm\u00e9es Marseille France"},{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ, Universit\u00e9 di Corsica, IRD 190, Inserm 1207, IRBA)  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Leparc\u2010Goffard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de virologie Institut de Recherche Biom\u00e9dicale des Arm\u00e9es Marseille France"},{"name":"Centre National de R\u00e9f\u00e9rence des Arbovirus Institut de Recherche Biom\u00e9dicale des Arm\u00e9es Marseille France"},{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ, Universit\u00e9 di Corsica, IRD 190, Inserm 1207, IRBA)  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franck","family":"de Laval","sequence":"additional","affiliation":[{"name":"Service de Sant\u00e9 des Arm\u00e9es, CESPA Centre d'\u00e9pid\u00e9miologie et de sant\u00e9 publique des arm\u00e9es Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Fernandes\u2010Pellerin","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Clinical Research Coordination Office Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Claude","family":"Manuguerra","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks (ERI) Unit Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e9lix","family":"Rey","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Unit\u00e9 de Virologie Structurale Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milena","family":"Hasan","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Single Cell Biomarkers UTechS Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyril","family":"Badaut","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Virus Emergents (UVE: Aix\u2010Marseille Univ, Universit\u00e9 di Corsica, IRD 190, Inserm 1207, IRBA)  France"},{"name":"Unit\u00e9 de virologie Institut de Recherche Biom\u00e9dicale des Arm\u00e9es, Br\u00e9tigny\u2010sur\u2010Orge  France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Flamand","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Unit\u00e9 de Virologie Structurale Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Matheus","sequence":"additional","affiliation":[{"name":"Institut Pasteur Universit\u00e9 Paris Cit\u00e9, Laboratory for Urgent Response to Biological Threats (CIBU), Environment and Infectious Risks (ERI) Unit Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8872-1774","authenticated-orcid":false,"given":"S\u00e9bastien","family":"Briolant","sequence":"additional","affiliation":[{"name":"D\u00e9partement Microbiologie et maladies infectieuses, Institut de Recherche Biom\u00e9dicale des Arm\u00e9es (IRBA) Unit\u00e9 Parasitologie et Entomologie Marseille France"},{"name":"Aix Marseille Universit\u00e9, SSA, AP\u2010HM Marseille France"},{"name":"IHU M\u00e9diterran\u00e9e Infection Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(52)90042-4"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0805715"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00625-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2110.151125"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)00562-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1601824"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiab173"},{"key":"e_1_2_9_9_1","volume-title":"Statement on the First Meeting of the International Health Regulations (2005) (IHR 2005). Emergency Committee on Zika Virus and Observed Increase in Neurological Disorders and Neonatal Malformations","author":"World Health Organization","year":"2016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011651"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1053-1057.2000"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.22.13924-13933.2005"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21091"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-101416-041848"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1684\/vir.2024.1050"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00229-06"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.01.002"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan1589"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03449-7"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13091771"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1403.070906"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002311"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1612600"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2311.170337"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2018.09.015"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy261"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.17.11403-11411.2005"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzr058"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/216164a0"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083567"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e21945"},{"key":"e_1_2_9_32_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2022"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1641"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix472"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2023.11.016"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16231-6"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw589"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix967"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/0470058005.ch17"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00354-15"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiae299"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19096-y"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.01.036"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz331"}],"container-title":["Journal of Medical Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jmv.70368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:38:12Z","timestamp":1754905092000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jmv.70368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,22]]},"references-count":43,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/jmv.70368"],"URL":"https:\/\/doi.org\/10.1002\/jmv.70368","archive":["Portico"],"relation":{},"ISSN":["0146-6615","1096-9071"],"issn-type":[{"value":"0146-6615","type":"print"},{"value":"1096-9071","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,22]]},"assertion":[{"value":"2025-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70368"}}