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There is a pressing need for the development of a more convenient and expeditious detection strategy for hMPV. The present study focused on the expression and purification of the highly conserved nucleoprotein (N) of hMPV, which served as an antigen in the generation of specific nanobodies and mouse monoclonal antibodies. Subsequently, we evaluated the efficacy of these immunological reagents in detecting the hMPV antigen through the application of double\u2010antibody sandwich enzyme\u2010linked immunosorbent assay and colloidal gold lateral flow chromatography test strips. These detection methods were successfully utilized on the recombinant antigen, cell culture\u2010derived hMPV, and nasopharyngeal swab specimens. 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